旋毛虫成虫与肌幼虫排泄分泌抗原蛋白组分的比较分析  

Comparison of Protein Components of Excretory-Secretory Antigens of Adult Worm and Muscle Larvae of Trichinella spiralis

作　　者：马鸣旺[1] 张志兰 申丽洁[3] 董明治[3] 

机构地区：[1]山西医科大学汾阳学院生物化学教研室,山西汾阳032200 [2]山西省汾阳市中医院妇产科 [3]大理学院寄生虫学教研室,云南大理671000

出　　处：《中国生物制品学杂志》2010年第11期1201-1203,共3页Chinese Journal of Biologicals

摘　　要：目的比较大理猪源旋毛虫(Trichinela spiralis)成虫与肌幼虫排泄分泌抗原(Excretory-secretory antigens,ES)的蛋白组分差异,并进一步分析其反应原性,为分离筛选出免疫原性和反应原性强的旋毛虫抗原成分奠定基础。方法分别收集和纯化旋毛虫成虫、肌幼虫虫体,制备ES,采用SDS-PAGE分析成虫和肌幼虫ES蛋白成分,Western blot分析其反应原性。结果经SDS-PAGE分析,旋毛虫成虫ES显示17条蛋白条带,相对分子质量范围在120000～14000之间,其中主带6条,相对分子质量分别为120000、64000、43000、40000、35000、33000;旋毛虫肌幼虫ES显示20条蛋白条带,相对分子质量范围在112000～10000之间,其中主带11条,相对分子质量分别为112000、66000、56000、55000、53000、49000、45000、43000、25000、21000、10000。Westernblot分析表明,旋毛虫成虫ES抗原显示7条反应带,相对分子质量分别为43000、40000、35000、27000、19000、18000、14000,其中相对分子质量43000、40000、27000、18000的条带着色明显;旋毛虫肌幼虫ES抗原显示14条反应带,相对分子质量范围在74000～12000之间,其中相对分子质量53000、49000、45000、43000、35000、27000、18000、12000的条带显色明显。结论旋毛虫成虫和肌幼虫ES抗原蛋白组分均复杂,有共同组分,也有不同组分,旋毛虫ES抗原具有较强的反应原性,是旋毛虫病研究的重要候选抗原。Objective To compare the protein components of excretory-secretory（ES）antigens of adult worm and muscle larvae of Trichinella spiralis,analyze their reactogenicities and lay a foundation of separation and screening of Tirchinella spiralis antigen component with high immunogenicity and reactogenicity.Methods The adult worms and muscle larvae of Tirchinella spiralis were collected,with which ES antigens were prepared then analyzed for protein components by SDS-PAGE and for reactogenicities by Western blot.Results Seventeen protein bands with relative molecular masses of 120 000 ～ 14 000,including six main protein bands with relative molecular masses of 120 000,64 000,43 000,40 000,35 000 and 33 000 respectively were observed on the SDS-PAGE profile of adult worm of Trichinella spiralis.However,twenty protein bands with relative molecular masses of 112 000 ～ 10 000,including eleven main bands with relative molecular masses of 112 000,66 000,56 000,55 000,53 000,49 000,45 000,43 000,25 000,21 000 and 10 000 respectively were observed on the SDS-PAGE profile of muscle larvae.The ES antigen of adult worm showed seven reaction bands on Western blot profile,with relative molecular masses of 43 000,40 000,35 000,27 000,19 000,18 000 and 14 000 respectively,of which the ones with relative molecular masses of 43 000,40 000,27 000 and 18 000 were stained obviously.However,the ES antigen of muscle larvae showed fourteen bands on Western blot profile,with relative molecular masses of 74 000 ～ 12 000,of which the ones with relative molecular masses of 53 000,49 000,45 000,43 000,35 000,27 000,18 000 and 12 000 were stained obviously.Conclusion Both the protein components of adult worm and muscle larvae of Trichinella spiralis were complicated,including common and different components.ES antigen showed high reactogencity and might be used as an important candidate antigen for study on trichinelliasis.

关 键 词：旋毛虫病 成虫 肌幼虫 排泄分泌抗原 

分 类 号：R383.15[医药卫生—医学寄生虫学] R392.33[医药卫生—基础医学]