雏鸡不同组织TLR1、TLR2、TLR4、TLR5和TLR15 mRNA转录水平相对定量研究  

Relative Quantification of mRNA Transcription of Toll-like Receptors in Different Tissues of Chicken by Real-time Quantitative PCR

作　　者：周作勇[1,2] 聂奎[3] 宋振辉[1] 胡世君[1] 周荣琼[1] 郭智莉[3] 刘丽琼[3] 秦波[3] 

机构地区：[1]西南大学荣昌校区动物医学系,重庆402460 [2]西南大学蚕学与系统生物研究所,重庆400715 [3]西南大学动物科技学院,重庆400715

出　　处：《畜牧兽医学报》2010年第11期1453-1459,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：西南大学青年基金资助项目(QNRC200804)

摘　　要：参考GenBank登录的ChTLRs基因序列设计实时定量PCR特异性引物,建立检测鸡Toll样受体(ChTLR)mRNA相对转录水平的实时定量PCR方法,分析ChTLR1、ChTLR2、ChTLR4、ChTLR5和ChTLR15在雏鸡不同器官组织中的转录水平。结果显示5种ChTLRs在脾脏、法氏囊、胸腺和各段肠道组织中均有转录。其中,ChTLR1 mRNA在法氏囊、肾脏和盲肠组织中转录水平较高;ChTLR2 mRNA在脾脏、法氏囊和肝脏等组织中转录水平较高,在肾脏、肺脏和皮肤未检测到转录;ChTLR4 mRNA在所检测组织中转录水平差异较小,在脾脏、十二指肠和胸腺转录水平较高;ChTLR5 mRNA在肾脏、脾脏和空肠中的转录水平较高;ChTLR15 mRNA在法氏囊中转录水平最高,其次为脾脏和盲肠。本研究建立了检测ChTLRs mRNA在不同器官组织中表达水平的实时定量PCR方法,ChTLRs mRNA在雏鸡各器官组织中转录水平差异较大,可能与雏鸡各器官组织对病原的识别和抵抗能力有关。According to the sequences of chicken Toll-like receptors（ChTLR）,the real-time PCR primers were designed and synthesized to establish a method for the detection of mRNA transcription of ChTLRs by real-time quantitative PCR.And the transcriptions of ChTLRs in different tissues of Luo-man chicken were analyzed.The result showed that the ChTLRs mRNA were transcripted in spleen,bursa,thymus and intestinal tract.The ChTLR1 is transcripted at high level in bursa,kidney and cecum,in contrast,ChTLR2 mRNA is restricted in the partial tissues,and at high level in spleen,bursa and liver,and not detected in kidney,lung and skin.ChTLR4 is transcripted at approximately the same level in all tissues tested.The ChTLR5 is transcripted at high level in kidney,spleen and cecum,and ChTLR15 is transcripted at high level in bursa,spleen and cecum.These results indicated that the real-time quantitative PCR was established successfully for the detection of ChTLRs mRNA transcription,and there were comparatively large differences on the transcription level of ChTLRs mRNA in different tissues,which may be associated with the ability of chicken to recognize pathogens.

关 键 词：TOLL样受体 mRNA转录水平 罗曼鸡 荧光定量PCR 

分 类 号：S852.42[农业科学—基础兽医学]