内皮素受体B在不同毛色羊驼皮肤中的表达与定位  

Expression and Immunolocalization of Endothelin Receptor B in Alpaca Skin of Different Colors

作　　者：耿建军[1] 穆晓丽[1] 孙乐天[2] 姜俊兵[1] 张杰[1] 李宏全[1] 张映[1] 董常生[1] 

机构地区：[1]山西农业大学动物科技学院,太谷030801 [2]太原动物园兽医院,太原030009

出　　处：《畜牧兽医学报》2010年第11期1478-1484,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30571070);山西省自然科学基金(2007011082)

摘　　要：研究内皮素受体B(endothelin recepter B,EDNRB)在羊驼皮肤中的表达与定位,以及在不同毛色中的差异比较,探讨其在羊驼毛色形成中的作用及其相关机制。以不同毛色的成年羊驼为研究对象,用RT-PCR法检测EDNRB基因在不同毛色皮肤中的表达,并使用实时荧光定量PCR技术分析不同毛色羊驼皮肤EDNRB基因的相对表达量,采用Western blot法和免疫组织化学法对EDNRB在羊驼皮肤中的表达进行定位和半定量分析。荧光定量PCR结果显示棕色羊驼中EDNRB基因的相对表达量是白色羊驼的2.1651倍;Western blot结果显示EDNRB蛋白在棕色组的表达显著高于白毛组(P<0.05);免疫组化试验结果显示,EDNRB在羊驼皮肤的毛乳头、毛根鞘及表皮细胞中均有表达,且在棕色皮肤组织中显著表达(P<0.05)。试验结果提示,EDNRB与羊驼黑色素细胞的活动密切相关,并参与了羊驼毛色和肤色的形成。The aim of this experiment is to study the expression and immunolocalization of Endothelin receptor B in alpaca skin,to compare the expression quantity in different colors,and to explore the function of EDNRB in the process of coat color formation.The whole testes were obtained from adult alpaca of white and brown hair colors.The mRNA and protein expression level of EDNRB in alpaca skin of different colors were examined by immunohistochemistry,Real-time quantitative PCR and Western blot.The results showed that the EDNRB gene was expressed in alpaca skin reliably.The expressive quantity of EDNRB gene in the brown alpaca skin tissue was 2.165 1 times than that in white one.The distribution of EDNRB in alpaca skin were demonstrated,the expression was notable difference between the white and brown alpaca based on the average optical density.Our findings showed that EDNRB was closely related to the activity of alpaca melanocytes,and was involved in the formation of alpaca hair and skin color.

关 键 词：内皮素受体B 羊驼 皮肤 实时荧光定量PCR WESTERN BLOT 免疫组织化学 

分 类 号：Q786[生物学—分子生物学] S852.16[农业科学—基础兽医学]