中国11个地方鸡种中鱼腥味敏感基因FMO3基因型频率分布研究  

Genotype Frequencies of the Fishy Taint Susceptible Gene FMO3 in 11 Chinese Local Chicken Breeds

作　　者：王晓亮[1] 徐桂云[1] 郑江霞[1] 姚俊峰[1] 曲鲁江[1] 杨宁[1] 

机构地区：[1]中国农业大学动物科技学院畜禽育种国家工程实验室,北京100193

出　　处：《畜牧兽医学报》2010年第11期1497-1501,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家高技术研究发展计划(2006AA10A121;2008AA101009);国家重点基础研究发展计划(2006CB102102);国家科技支撑计划(2006BDA01A09;2008BADB2B06);国家蛋鸡产业技术体系;土鸡蛋生产关键技术研究与示范(nyhyzx07-039)

摘　　要：本试验旨在研究影响鸡蛋鱼腥味的FMO3基因T329S突变在11个中国地方鸡种中的分布。所选的11个地方鸡种包括:北京油鸡、河北柴鸡、固始鸡、溧阳鸡、如皋鸡、太湖鸡、淮南麻黄鸡、丝羽乌骨鸡、东乡绿壳蛋鸡、文昌鸡、藏鸡,采用PRC-RFLP方法对FMO3基因及基因型频率进行检测。试验结果表明东乡绿壳蛋鸡全部表现为TT基因型,不表现鱼腥味综合症,具有较好的蛋品质;而其他10个地方鸡种中均有SS基因型的鱼腥味综合症易感个体,其中北京油鸡SS基因型频率最高,为8.8%,藏鸡的SS基因型频率最低,为0.7%。地方鸡种中鱼腥味综合症易感基因型频率都不高,可以通过PCR-RFLP的方法予以剔除。The objective of the study was to detect the distribution of T329S mutation in 11 Chinese local chicken breeds.The FMO3 genotype frequency in 11 Chinese local chicken breeds including Beijing You,Hebei Chai,Gushi,Liyang,Rugao,Taihu,Huainan Mahuang,Silky,Dongxiang Blue-shelled,Wenchang and Tibetan chicken,were detected by PRC-RFLP method.The results showed that Dongxiang Blue-shelled chicken only had TT genotype,suggesting that no individual would lay fishy taint eggs in this breed.Fishy taint susceptible individuals with genotype SS were detected in all other 10 local breeds.Of these breeds,Beijing You chicken had the highest frequency of the SS genotype（8.8%） and Tibetan chicken present the lowest（0.7%）.In conclusion,the frequencies of fishy taint genotype in Chinese local chicken breeds were generally low and the susceptible individuals could be easily detected by PCR-RFLP method.

关 键 词：鱼腥味 中国地方鸡种 FMO3 PCR-RFLP 

分 类 号：S831.2[农业科学—畜牧学]