己烯雌酚对幼年香猪睾丸细胞的影响  

The Effect of DES on the Testis Cell of Xiang Pig

作　　者：田兴贵[1] 李江森[1,2] 主性[1] 冯会利[1] 刘若余[1] 

机构地区：[1]贵州大学动物科学学院,贵阳550025 [2]集美大学水产学院,厦门361021

出　　处：《畜牧兽医学报》2010年第11期1510-1514,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：贵州省科学基金(黔科合J字[2006]2031号);贵州大学博士基金(2005)资助

摘　　要：为了认识己烯雌酚(DES)对幼年雄性香猪睾丸生殖细胞和支持细胞数的影响,将20只20～25日龄幼年雄性香猪分成4组,对照组只注射生理盐水,试验组连续9d(每天1次)经腹腔分别注射不同剂量(0.03、0.3、3mg·kg-1)己烯雌酚后,最后一次注射24h后取左侧睾丸,入40g·L-1多聚甲醛固定24h,常规制备5μm石蜡切片,用免疫组织化学方法对睾丸支持细胞特异性蛋白生长转录因子4(GATA4)进行检测。显微镜下分别计数支持细胞和生殖细胞数,并统计分析。结果发现:己烯雌酚用量为0.3和3mg·kg-1能促使睾丸生殖细胞数显著增多(P<0.05);而使睾丸支持细胞数量显著减少(P<0.05);首次发现DES能促进幼年香猪睾丸生殖细胞增殖而抑制其支持细胞增殖。DES的作用因细胞类型不同而有所不同,但其机制还需进一步研究。The objective of this study was to explore the effects of diethylstilbestrol daily exposure on testis Sertoli cells and germ cells of immaturity Guizhou Xiang-Pig.Twenty male Xiang pig,20-25 days old,were divided into four groups（one control group and three test groups） of 5 each.Test group 1,2 and 3 was received different doses,0.03,0.3 and 3 mg·（kg-1·d-1）,of diethylstilbestrol for 9 days by intraperitoneal respectively.24 h after the last injection,the left testis was removed and fixed in 40 g·L-1 paraformaldehyde for 24 h,and then was processed to paraffin wax.Five-micron-thick sections were cut.We used an immunohistochemical method with antibody against GATA-4,a special protein of Sertoli cell,to determine the Sertoli cells,the number of Sertoli and germ cells were counted under microscope.The result showed that DES significantly（P0.05） increased the germ cell number,but significantly（P0.05） decreased Sertoli cell number at 0.3,3（mg·kg-1·d-1） dose,no significance was found at 0.03 mg·（kg-1·d-1） dose of DES（P0.05）,compared with that in control group.DES could promote the germ cell proliferation of immature xiang pig testes,while decrease the Sertoli cell proliferation.It was concluded that the DES had different effects on different cell types.

关 键 词：己烯雌酚 香猪 睾丸 支持细胞 生殖细胞 

分 类 号：Q492.4[生物学—生理学]