吸烟的慢性阻塞性肺病患者外周血中性粒细胞Bak mRNA的表达  

Bak mRNA expression in circulating neutrophils in smokers with chronic obstractive pulmonary disease

作　　者：张冀松[1] 夏敬文[1] 陈小东[1] 

机构地区：[1]复旦大学附属华山医院呼吸科,上海200040

出　　处：《复旦学报（医学版）》2010年第6期657-661,共5页Fudan University Journal of Medical Sciences

基　　金：上海市科学技术委员会基金项目(074119508)

摘　　要：目的探讨吸烟的慢性阻塞性肺病(chronic obstructive pulmonary disease,COPD)患者外周血Bak基因表达异常及其与COPD发病的关系。方法入选者包括健康对照组(不吸烟者10例、吸烟者14例)和轻中度吸烟COPD患者9例。使用Percoll非连续密度梯度沉降法分离人外周血中性粒细胞,使用Real-time PCR来检测外周血中性粒细胞中BakmRNA的表达水平。结果相对于健康对照组,轻中度COPD患者外周血中性粒细胞中的BakmRNA水平(0.88±0.38)明显降低,差异有统计学意义(P<0.05);吸烟健康者外周血中性粒细胞中BakmRNA水平(1.23±0.29)低于不吸烟健康者(1.33±0.38),但差异无统计学意义;BakmRNA水平与FEV1/预计值%、FEV1/FVC%均呈正相关,相关系数r分别为0.438 8(P<0.05)和0.432 1(P<0.05)。结论 Bak基因表达异常可能与COPD发病有一定关系。BakmRNA水平与临床肺功能之间有明显相关性,推测可能与COPD的严重程度相关。Objective To investigate the relationship between abnomal expression of Bak gene in peripheral blood in chronic obstructive pulmonary disease（COPD） smokers and the pathogenesis of COPD.Methods We included 10 healthy non-smokers,14 healthy smokers and 9 mild to moderate COPD smokers.Human peripheral blood neutrophils were isolated by using Percoll discontinuous density gradient sedimentation.And we detected mRNA levels of Bak gene in peripheral blood neutrophils by using Real-time PCR.Results Levels of Bak mRNA in circulating neutrophils of mild to moderate COPD smokers decreased statistically compared to healthy subjects（P0.05）.Levels of Bak mRNA in healthy smokers were lower than in that of healthy non-smokers without significant difference（P0.05）.Bak mRNA levels and FEV1/FEV1 pred% were positively correlated,as well as Bak mRNA levels and FEV1/FVC%.Conclusions There is possible relationship between abnormal expression of Bak gene and pathogenesis of COPD.In addition,levels of Bak mRNA might be related to the severity of COPD.

关 键 词：慢性阻塞性肺疾病 吸烟 BAK基因 中性粒细胞凋亡 

分 类 号：R563[医药卫生—呼吸系统]