桃叶卫矛组织培养的研究  

Study of Tissue Culture of Euonymus bungeanus

作　　者：张丽杰[1] 王强恩[2] 周强[2] 张才[2] 

机构地区：[1]沈阳农业大学林学院,辽宁沈阳110161 [2]辽宁省实验林场,辽宁清原113311

出　　处：《北方园艺》2010年第17期141-143,共3页Northern Horticulture

基　　金："十一五"国家科技基础条件平台建设资助项目(2005DKA21003)

摘　　要：以桃叶卫矛的成熟胚为外植体,研究了不同消毒方法对桃叶卫矛离体胚萌发的影响,并筛选出最佳消毒液及消毒时间。同时,选取不同培养基和激素浓度进行卫矛的离体胚培养最佳激素组合筛选。结果表明:不同的灭菌剂和不同的灭菌时间对无菌苗的萌发与生长有显著的影响;筛选出MS1/2+BA0.5mg/L+NAA0.2mg/L+蔗糖30g/L+琼脂7g/L的培养基为成熟胚萌发的最适培养基配比。In order to achieve the exsomatize regeneration of the Euonymus bungeanus Maxim.taking its mature embryo as explants,studies the influence on the explants growth under different sterilizing agents and different sterilization time,and impact on them which was caused by different mediums and different hormone concentration ratios.This experiment studies the possible approaches of from vitro germination to normal strains with the hope to get the regeneration plants.The results showed that the different agents and different sterilization sterilization time on the germination and growth of non-vaccine has a significant impact.Filter out the MS1/2＋BA 0.5mg/L＋NAA 0.2mg/L＋sucrose 30g/L＋agar 7 g/L medium for the germination of mature embryos of the best medium ratio.

关 键 词：桃叶卫矛 灭菌方法 组织培养 

分 类 号：S687[农业科学—观赏园艺]