海水胁迫下单叶蔓荆基因差异表达的初步分析  

Preliminary Analysis of Gene Expression in the Response to Seawater in the Vitex trifolia Linn.var.simplicifolia Cham

作　　者：张家福[1] 关洪斌[1] 位建业[1] 史源[1] 王卫飞[1] 

机构地区：[1]山东大学威海分校海洋学院,山东威海264209

出　　处：《北方园艺》2010年第17期152-154,共3页Northern Horticulture

摘　　要：应用cDNA-AFLP技术,以单叶蔓荆为研究对象,对海水胁迫下单叶蔓荆叶片基因表达进行了分析。结果表明:通过256对引物组合的筛选,共分离得到45个差异表达的转录衍生片段(TDF),随机选取其中10个差异片段进行测序,并进行Blast-x分析。Blast-x分析获得5个与已知片段知基因有较高的同源性,主要包括转录因子、与离子转运有关的蛋白、以及胁迫相关蛋白等的编码基因。并构建了单叶蔓荆在海水胁迫下的基因表达谱,从基因组水平上识别了一批受盐胁迫诱导或抑制表达、与耐盐相关的基因,这些新基因可用于耐盐的分子机理研究。Gene The expression in the response to seawater in the Vitex trifolia Linn.var.simplicifoliaCham was analyzed by the technology of cDNA-AFLP.The results showed that two hundred and fifty-six primer combinations were used to investigate,fouty-five differentially expressed cDNAs were found.Gene expression profiling in response to seawater and differentially expressed genes of Vitex trifolia Linn.var.simplicifolia Cham were revealed and identified via cDNA-AFLP analysis.This work begins to reveal potential molecularmetablosim for salt tolerance of mangrove.

关 键 词：单叶蔓荆 海水胁迫 差异表达 CDNA-AFLP 

分 类 号：Q943.2[生物学—植物学]