17β-雌二醇对子宫内膜异位症患者在位内膜间质细胞趋化因子SDF-1/CXCR4 mRNA及蛋白表达的影响  

Effect of 17β-estradiol on expressions of SDF-1/CXCR4 mRNA and protein in eutopic endometrium of patients with endometriosis

作　　者：廉红梅[1] 刘义[2] 吕立群[1] 

机构地区：[1]湖北省妇幼保健院妇科,湖北武汉430070 [2]华中科技大学同济医学院附属协和医院妇产科

出　　处：《中国妇幼保健》2010年第33期4914-4917,共4页Maternal and Child Health Care of China

基　　金：国家自然科学基金项目〔30750007〕

摘　　要：目的:研究17β-雌二醇(17β-E2)对子宫内膜异位症(内异症)患者在位子宫内膜间质细胞趋化因子SDF-1及其受体CXCR4 mRNA及蛋白表达的影响,探讨SDF-1/CXCR4在介导雌激素促进内异症发生发展中的作用。方法:体外分离、培养内异症患者在位子宫内膜间质细胞。用不同浓度17β-E2(10-12、10-10、10-8、10-6mol/L)处理子宫内膜间质细胞12 h、24 h、48 h,利用实时定量PCR(RQ-PCR)技术和流式细胞检测技术分别检测17β-E2处理前后子宫内膜间质细胞趋化因子SDF-1/CXCR4 mRNA及蛋白的表达;同法分析雌激素受体拮抗剂IC I182780(10-8mol/L)对SDF-1/CXCR4 mRNA和蛋白表达的影响。结果:不同浓度17β-E2作用于内异症患者在位子宫内膜间质细胞12 h、24 h、48 h后,SDF-1/CXCR4 mR-NA及蛋白的表达呈不同程度增高,于10-10mol/L作用最明显。雌激素受体拮抗剂IC I182780能明显抑制17β-E2的作用。结论:雌激素可促进内异症患者在位子宫内膜间质细胞SDF-1/CXCR4 mRNA及蛋白的表达,SDF-1/CXCR4可能参与了内异症的形成和发展。Objective： To research the effect of 1713 - estradiol on expressions of SDF - 1 and its receptor CXCR4 mRNA and protein in eutopic endometrium of patients with endometriosis, explore the function of SDF - 1/CXCR4 in occurrence and development of endo- metriosis. Methods： Stromal cells of eutopie endometrium in patients with endometriosis were separated and cultured in vitro, then they were treated by 17β - estradiol of various concentrations （ 10 -12, 10 -10, 10 -8, 10 -6 mol/L） for 12, 24 and 48 hours, real - time quantitative PCR （ RQ - PCR） and flow cytometry were used to detect the expression levels of SDF - 1/CXCR4 mRNA and protein before and after treat- ment with 17β - estradiol; the effect of estrogen receptor antagonist ICI182780 （ 10 -8 mol/L） on expressions of SDF - I/CXCR4 mRNA and protein was analyzed. Results： After treating stromal cells of eutopic endometrium in patients with endometriosis by 17β - estradiol of various concentrations for 12, 24 and 48 hours, the expression levels of SDF - 1/CXCR4 mRNA and protein increased to varying degrees, the peak appeared when the concentration was 10-10 rnol/L; estrogen receptor antagonist ICI182780 inhibited the expression of 17β-estradiol obviously. Conclusion： Estrogen can promote the expressions of SDF- 1/CXCR4 mRNA and protein in eutopic endometrium of patients with endometriosis, SDF - 1/CXCR4 may participate in the occurrence and development of endometriosis.

关 键 词：子宫内膜异位症 趋化因子 子宫内膜间质细胞 雌激素SDF-1 CXCR-4 

分 类 号：R711.71[医药卫生—妇产科学]