C/P对EBPR系统PAOs与GAOs竞争及PHAs代谢过程影响研究  

Effects of C/P on the Competition Between PAOs and GAOs and PHAs Metabolism in EBPR System

作　　者：蒋涛[1] 方婧[1] 孙培德[1] 钟晓[1] 徐少娟[1] 方治国[1] 

机构地区：[1]浙江工商大学环境科学与工程学院,杭州310012

出　　处：《环境科学》2010年第12期2938-2944,共7页Environmental Science

基　　金：浙江省自然科学基金重点项目(Z507721);浙江省教育厅项目(Y200907043;Y200804075)

摘　　要：以富含聚磷菌(PAOs)活性污泥为基础,基于FISH技术研究了SBR工艺不同C/P(25∶1、20∶1、15∶1和10∶1)对EBPR系统中功能菌变化特征与微生物胞内聚合物(PHAs)代谢过程的影响.结果表明,经过10 d运行处理,C/P分别为25∶1、20∶1和15∶1系统磷酸盐去除率>88%,而C/P为10∶1系统磷酸盐的去除率为0%.FISH检测结果显示,随着C/P从25∶1下降到10∶1,EBPR系统中PAOs的含量相应从(76.42±1.24)%减少到(10.40±0.97)%,而聚糖菌(GAOs)则从(16.36±3.41)%增加到(34.25±2.59)%.在厌氧段,不同C/P条件下EBPR系统中PHB和PHV的合成动力学系数大小分别为K25∶1>K20∶1>K15∶1>K10∶1和K15∶1>K20∶1>K25∶1>K10∶1.随着C/P从25∶1下降到10∶1,合成PHB在PHAs中所占的比例从85%下降到24%,而PHV则从15%上升到76%;在好氧段,不同C/P系统消耗PHB和PHV的动力学系数大小均为K20∶1>K25∶1>K15∶1>K10∶1,且C/P为25∶1、20∶1和15∶1时系统消耗主要成分是PHB(占PHAs 71%～75%),而C/P为10∶1时系统消耗主要成分是PHV(占PHAs 71%).由此表明,随着C/P的降低,EBPR系统内GAOs增加而PAOs减少,从而导致系统内PHB合成与消耗比例逐渐减少,而PHV合成与消耗比例逐渐增加.Based on accumulating enrich phosphorus accumulating organisms（PAOs） in activated sludge and by fluorescence in situ hybridization（FISH） technique,the effects of C /P（25 ∶ 1,20 ∶ 1,15 ∶ 1 and 10 ∶ 1） on flora changes and polyhydroxyalkanoates（PHAs） metabolism in enhanced biological phosphorus removal（EBPR） system were systematically studied.The results show that after 10 days treatment（acetate as carbon source）,the systems with C /P of 25∶ 1,20∶ 1 and 15∶ 1 have good phosphate removal rate（ 〉88%）;while for C/P of 10 ∶ 1 system,the phosphate removal rate was 0%.FISH results showed that the content of PAOs decreased from（76.42 ± 1.24）% to（10.40 ± 0.97）% with C /P decreasing from 25 ∶ 1 to 10 ∶ 1,while glycogen accumulating organisms（GAOs） increased from（16.36 ± 3.41）% to（34.25 ± 2.59）%.In various C /P systems in the anaerobic,the production kinetic coefficients of PHB and PHV were K25∶1〉 K20∶1 〉K15∶1 〉K10∶1 and K15∶1〉 K20∶1 .K25∶1 〉K10∶1,respectively.The PHB production in the proportion of PHAs decreased from 85% to 24%,while PHV increased from 15% to 76% with C /P decreasing from 25∶ 1 to 10∶ 1.For various C /P systems in the aerobic phase,their consumption kinetic coefficients of both PHB and PHV were K20∶1 〉K25∶1 〉K15∶1〉 K10∶1.PHB（the proportion of PHAs is 71%-75%） was the main consumption component in C /P of the 25∶ 1,20∶ 1 and 15∶ 1 systems;while PHV（the proportion of PHAs is 71%） was the main consumption component in C /P of 10∶ 1 system.It is suggested that an increases of GAOs and decreases of PAOs in the EBPR system lead to the decreasing of PHB production and consumption,and to the increasing of PHV.

关 键 词：生物强化除磷(EBPR) 聚磷菌(PAOs) 聚糖菌(GAOs) 聚羟基烷酸(PHAs) 荧光原位杂交技术(FISH) 

分 类 号：X703[环境科学与工程—环境工程]