AFLP技术在玉米自交系类群分析研究中的应用  

Genetic Diversity Analysis of Maize Inbred Lines with AFLP Technique

作　　者：贾建航[1] 李传友[1] 金德敏[1] 王斌[1] 

机构地区：[1]中国科学院遗传研究所

出　　处：《高技术通讯》1999年第4期43-47,共5页Chinese High Technology Letters

基　　金：国家"九五"科技攻关资助项目

摘　　要：用ＡＦＬＰ分子标记技术对２３个应用较广泛的玉米自交系进行了遗传多样性分析。在ＡＦＬＰ分析当中，共采用了７个ＥｃｏＲＩ／ＭｓｅＩ引物组合，每个引物组合扩增出的条带数在５５—９６条之间。利用以上ＡＦＬＰ数据进行了聚类分析，将２３个玉米自交系分为３个群。结果表明，ＡＦＬＰ聚类图基本与系谱分析一致。ＡＦＬＰ技术是检测玉米自交系遗传多样性有效的分子标记技术。另外通过ＡＦＬＰ分析，找到了三个玉米自交系的特异ＡＦＬＰ产物。AFLP was used as a DNA fingerprinting technique in 23 maize inbred lines analysis. A dendrogram based on AFLP markers was achieved and compared with the pedigree relationship of these inbred lines. Seven primer combinations were used in the AFLP analysis and 55 to 96 bands were in amplified each lane. Through cluster analysis, three groups were clearly identified, which correspond to pedigree relationship. The results show that AFLP marker is the most effective molecular marker to identify the germplasm and might be used in germplasm identification.

关 键 词：AFLP 聚类分析 遗传多样性 种质 玉米 自交系 

分 类 号：S513.032[农业科学—作物学]