中华眼镜蛇毒活性组分抑制微血管形成研究  

Inhibitory effect of China cobra venom active factor on neovascularization

作　　者：朱柳[1] 余清声[1] 刘新艳[1] 余红娥[1,2] 袁牧[1] 王桂平[1] 楼晓华[3] 滕脉坤[3] 

机构地区：[1]广州医学院药物研究中心,广东广州510182 [2]广东药学院,广东广州510006 [3]中国科学技术大学生命科学学院,安徽合肥230026

出　　处：《中国生化药物杂志》2010年第6期364-368,共5页Chinese Journal of Biochemical Pharmaceutics

基　　金：广东省自然科学基金资助项目(No.980463);卫生部科技基金课题资助项目(No.98-2-349);广州市教育局市属高校科技计划项目(No.1016)

摘　　要：目的探讨中华眼镜蛇毒活性组分(CCVAF)抗血管生成的作用。方法采用原代培养牛肺主动脉内皮细胞克隆形成实验、Transwell小室趋化实验及大鼠胸主动脉环体外无血清培养微血管样结构形成实验,分别观察CCVAF对内皮细胞克隆形成、细胞迁移及大鼠动脉环微血管生成的影响。结果 CCVAF浓度为1.25,2.5,5.0μg/mL时,其对内皮细胞克隆形成抑制率分别为17.7%,40.3%,62.9%,呈浓度依赖性(r=0.982,P<0.05),而对内皮细胞迁移抑制率分别为20.5%,59.0%,73.5%,呈浓度依赖性(r=0.902,P<0.05);大鼠动脉环培养至第13天,CCVAF组较溶剂对照组微血管生成率下降了86.5%,CCVAF+VEGF组较VEGF对照组微血管生成率下降了88.1%。结论 CCVAF能够抑制内皮细胞克隆的形成、Hela细胞诱导的内皮细胞迁移和大鼠动脉环微血管的生成。Purpose To investigate the antiangiogenesis effect of China cobra venom active factor（CCVAF）from China cobra venom.Methods Cell colony formation experiment,transwell chamber assay and the experiment of the growth of microvessels in serum free matrix culture of rat aorta were adopted to evaluate the effect of CCVAF on bovine arteria pulmonalis vascular endothelial cells（BAVEC）colony formation,migration and neovascularization.Results CCVAF inhibited the clone formation and migration of BAVEC in dose-dependent maner.The inhibition rates of clone formation and migration of different dose of CCVAF（ie.1.25,2.5 and 5.0 μg/mL）on BAVEC were 17.7%,40.3%,62.9%（r=0.982,P〈0.05）and 20.5%,59.0%,73.5%（r=0.902,P〈0.05）respectively.Compared with solvent control group,the inhibition rate of new capillaries sprouted from rat aortic ring of CCVAF was 86.5%,and compared with VEGF control group,the inhibition rate of（VEGF＋CCVAF）group was 88.1% on 13th day.Conclusion CCVAF can inhibit the colony formation,and migration of BAVEC and can inhibit the neovascularization of rat aortic ring.

关 键 词：眼镜蛇毒 细胞迁移 动脉环体外培养 血管生成 

分 类 号：R996.3[医药卫生—毒理学] R285[医药卫生—药学]