双辛可宁酸法测定胎盘多肽注射液中低浓度多肽的含量  

Determining the protein content of low concentration solution in placenta polypeptide injection by BCA protein method

作　　者：张云楚[1] 纪宇[1] 

机构地区：[1]江苏省食品药品检验所,江苏南京210008

出　　处：《中国生化药物杂志》2010年第6期402-404,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家药典委员会注射剂质量标准提高项目

摘　　要：目的建立双辛可宁酸(BCA)法测定胎盘多肽注射液低浓度多肽含量的方法。方法研究BCA法对胎盘多肽注射液低浓度多肽含量测定的可行性,并将其与Folin-酚法、双缩脲法、定氮法进行比较。结果 BCA法对牛血清白蛋白在19.2～115.2μg/mL的浓度范围内有良好的线性关系(r≥0.999 0),平均加样回收率为102.2%,RSD为3.8%。结论此法简便、快速、准确,适用于胎盘多肽注射液低浓度多肽的含量测定。Purpose To establish a method to determine the content of low concentration protein solution in placenta polypeptide injection.Methods The feasibility of BCA protein quantification method for low concertion protein was investigated and compared with Folin-phenol method,Biuret method and Kjeldahl method.Results The linear range of albumin bovine was 19.2-115.2 μg/mL（r≥0.999 0）.The average recovery of Albumin bovine was 102.2%（RSD 3.8%）.Conclusion This method is efficient,accurate and suitable for the assay of low concentration protein solution in placenta polypeptide injection.

关 键 词：胎盘多肽注射液 双辛可宁酸法 含量测定 

分 类 号：R927.2[医药卫生—药学]