不同接种方式下球孢白僵菌对小菜蛾的致病力  

Pathogenicity of Beauveria bassiana to diamondback moth by using different inoculation methods

作　　者：雷妍圆[1] 吕利华[2] 何余容[1] 

机构地区：[1]华南农业大学资源环境学院,广州510642 [2]广东省农业科学院植物保护研究所,广州510640

出　　处：《植物保护》2010年第6期142-146,共5页Plant Protection

基　　金：公益性行业(农业)科研专项(200803001);广东省农业攻关项目(2006B20301037)

摘　　要：本文比较了4种接种方式下球孢白僵菌Bb02菌株对小菜蛾2龄和3龄幼虫的致病力。当孢子悬浮液浓度为1×107个/mL时,球孢白僵菌对小菜蛾2龄幼虫校正死亡率为浸虫法73.79%,涂抹法68.28%,喷雾法51.72%和浸叶法33.79%;对3龄幼虫的校正死亡率为喷雾法57.93%,浸虫法57.24%,涂抹法55.86%和浸叶法28.28%,虽然浸虫法的校正死亡率稍低于喷雾法,但两者差异不显著。同时,2龄和3龄幼虫以浸虫法处理僵虫率最高,分别达31.25%和29.55%;致死速度最快,LT50分别为3.62 d和4.63 d。认为浸虫法最适于室内准确评价球孢白僵菌对小菜蛾的致病力。The pathogenicity of Beauveria bassiana strain Bb02 against the second and third instar larvae of the diamondback moth, Plutella xylostella L., was investigated by using four inoculation methods in the laboratory. It was shown that the mortality of larvae varied with different inoculation methods at a concentration of 1 × 10^7 conidia/mL suspension. The highest mortality 73.79% of second instar larvae was obtained by insect-immersing method, compared to other inoculation method （68.28% by insect-smearing, 51.72% by insect-spraying and 33.79% by cabbage leaf-immersing）. However, there were no significant differences between the highest mortali- ty 57.93% by insect-spraying method and 57.24% by insect-immersing method, when third instar larvae was provided. Insect-immersing method had also resulted in the highest total cadaver rates （31.25% of second instar larvae and 29.55% of third instar larvae） and the shortest LT50 values （3.62 days of second instar larvae and 4.63 days of third instar larvae） after treatment in the bioassay. It was indicated that insect-immersing method was the most effective inoculation method in the bioassay in assessing the pathogenicity of B. bassiana against the diamondback moth.

关 键 词：球孢白僵菌 小菜蛾 接种方式 致病力 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]