牡丹皮免煎颗粒与饮片有效成分含量的差异研究  

Study of Effective Content of Paeonia Compared Fried Free Particles and Pieces

作　　者：王晓琳[1] 张平[2] 郭敏[1] 

机构地区：[1]甘肃省中医院,甘肃兰州730050 [2]甘肃省药品检验所

出　　处：《甘肃中医》2010年第12期43-44,共2页Gansu Journal of Traditional Chinese Medicine

摘　　要：目的:比较中药牡丹皮免煎颗粒与其饮片中有效成分含量差异。方法:采用高效液相色谱法(HPLC),伊利特C18柱,以甲醇-水(50:50)为流动相,流速1.0 mL/min,检测波长274 nm。结果:牡丹皮免煎颗粒中丹皮酚含量为4.65 mg/g,牡丹皮中药饮片中丹皮酚含量为24.23 mg/g;丹皮酚在55～275μg范围内呈良好的线性关系,平均回收率为97.10%,RSD为0.7%(n=6)。结论:中药牡丹皮免煎颗粒与其饮片所含丹皮酚含量差异较大。Objective：To compare the difference of effective component paeonia between fried free particles and its pieces.Methods：To test with high performance liquid chromatography（HPLC）,Erie C18 column,with methanol-water（50：50） as mobile phase,velocity：1.0 mL/min,wavelength：274.Results：The content of polyphenol was 4.65 mg/g in fried free particles,24.23 mg/g in pieces.The polyphenol was linear relationship in range 55～275 μg,the average recovery was 97.10%,RSD for 0.7%,respectively.Conclusion： It was remarkable difference of content of polyphenol between fried free particles and its pieces.

关 键 词：免煎颗粒 丹皮酚 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]