应用姜黄素预防大鼠椎板切除术后硬膜外粘连的实验研究  

Experimental study of the preventive effect of curcumin on epidural adhesion after laminectomy in rats

作　　者：王立新[1] 张伟[1] 程兆明[1] 倪进荣[1] 

机构地区：[1]连云港市第二人民医院(蚌埠医学院附属连云港医院)骨科,江苏连云港222023

出　　处：《生物骨科材料与临床研究》2010年第6期1-4,共4页Orthopaedic Biomechanics Materials and Clinical Study

摘　　要：目的观察局部应用姜黄素预防椎板切除术后硬膜外粘连的效果。方法 36只SD大鼠随机分为3组,切除L1或L2椎板。术中各组分别以胶原蛋白海绵姜黄素(C组)、胶原蛋白海绵(B组)置于裸露的硬脊膜后,空白对照组(A组)不作治疗。术后第4周处死动物取材,分别作肉眼观察、羟脯氨酸(Hydroxyproline HOP)含量测定、胶原组织面积测定及成纤维细胞记数。结果 A组标本硬膜外瘢痕组织致密,与硬脊膜形成紧密粘连。C组无明显硬膜外粘连,HOP含量、胶原组织面积及成纤维细胞记数显著减少。B组HOP含量、胶原组织面积及成纤维细胞记数不同程度的减少,但不能避免硬膜外粘连的形成。结论局部应用姜黄素能够有效减少硬膜外瘢痕组织增生,避免椎板切除术后硬膜外瘢痕粘连。Objective To investigate the preventive effect of topical application of curcumin on epidural adhesion after laminectomy.Methods 36 SD rats were randomly divided into three groups,of which all of the L1 laminas were resected.The curcumin collagen sponge and collagen sponge were placed behind the exposed dura in group C and group A respectively.Group A was the control group.Animals were sacrificed after the fourth week,The content of hydroxyproline （HOP）,the area of collagen and the number of gibroblast cells were determined respectively after the naked eye obsebation.Results The epidural scar tissue was dense and had formed tight adhesion with the spinal dura mater in group A.No epidural adhesion was detected and the content of hydroxyproline （HOP）,the area of collagen and the number of gibroblast cells had been significantly reduced in group C.In group B,the content of hydroxyproline （HOP）,the area of collagen and the number of gibroblast cells had been reduced to varying degrees,while the epidural adhesion could not be prevented.Conclusion The topical application of curcumin can reduce the formation of epidural scar tissue and prevent epidural scar adhesion after laminectomy.

关 键 词：椎板切除术 硬膜外 粘连 姜黄素 胶原蛋白海绵 

分 类 号：R687.3[医药卫生—骨科学]