海洋真菌链孢粘帚菌(Gliocladium catenulatum T31)抗肿瘤活性代谢产物  

Antitumor Metabolites from Marine-Derived Fungus Gliocladium catenulatum T31

作　　者：任虹[1] 曹学丽[1] 顾谦群[2] 

机构地区：[1]北京工商大学化学与环境工程学院,植物资源研究开发北京市重点实验室,北京100048 [2]中国海洋大学医药学院,山东青岛266003

出　　处：《中国药学杂志》2010年第22期1720-1723,共4页Chinese Pharmaceutical Journal

基　　金：国家自然基金资助(30973631;30572279)

摘　　要：目的阐明海洋真菌链孢粘帚菌（Gliocladium catenulatum T31）的抗肿瘤活性次级代谢产物。方法摇床发酵培养生产菌T31,活性跟踪分离纯化T31发酵液中的活性单体化合物,并根据理化性质和光谱分析（ESI MS、UV、IR、NMR等）鉴定单体化合物结构;采用细胞形态镜检、MTT方法评价单体化合物对人类慢性髓性白血病K562细胞的抗肿瘤活性。结果从链孢粘帚菌（Gliocladium catenulatum T31）发酵液中分离并鉴定了7个单体化合物,分别为3个甾醇类化合物ergosta-5,7,22-triene-3β-ol（1）、ergosta-6,22-diene-3β-ol（2）、ergosterol peroxide（3）和四个蒽醌类化合物emodin（4）、citreorosein（5）、isorhodopti-lometrin（6）和lunatin（7）,化合物1-7对K562细胞显示不同程度的细胞增殖抑制活性,其中,化合物4-7对K562细胞的IC50分别为1.09、1.24、13.6和8.92μmol.L-1。结论海洋真菌链孢粘帚菌（Gliocladium catenulatumT31）的主要抗肿瘤活性次级代谢物是甾醇类和蒽醌类化合物。OBJECTIVE To investigate the antitumor metabolites from marine-derived fungus Gliocladium catenulatum T31.METHODS The producing strain T31 was fermented in a rotary shaker and the bioactive metabolites in the fermentation broth were isolated through a bioassay-guided separation procedure.The structures of the bioactive compounds were determined with physicochemical and spectroscopic data.The antitumor activity against K562 cell line in vitro was assayed by MTT method,accompanied with the cell morphological observation under the light microscope.RESULTS Seven compounds were isolated from the fermentation broth of Gliocladium catenulatum T31 and identified as three sterol derivatives including ergosta-5,7,22-triene-3β-ol（1）,ergosta-6,22-diene-3β-ol（2）,ergosterol peroxide（3）and four anthraquinones including emodin（4）,citreorosein（5）,isorhodoptilometrin（6）and lunatin（7）.Compound 1-7 inhibited the proliferation of K562 cells in various degrees,and compound 4-7 inhibited the growth of K562 cells with the IC50 values of 1.09,1.24,13.60 and 8.92 μmol·L-1,respectively.CONCLUSION The predominant antitumor metabolites from Gliocladium catenulatum T31 were sterol and anthraquinone derivatives.

关 键 词：链孢粘帚菌 活性跟踪 甾醇类 蒽醌类 抗肿瘤活性 

分 类 号：R282.77[医药卫生—中药学]