浙江省1998-2009年H3N2亚型流感流行株基因组全序列分析  

Study on the genome sequence of influenza virus subtype A/H3N2 strains circulated in Zhejiang province during 1998 to 2009

作　　者：赵斐斐[1] 卢亦愚[2] 冯燕[2] 徐昌平[2] 茅海燕[2] 周敏[2] 莫世华[2] 

机构地区：[1]宁波大学医学院,315211 [2]浙江省疾病预防控制中心

出　　处：《中华流行病学杂志》2010年第12期1368-1373,共6页Chinese Journal of Epidemiology

摘　　要：目的 从基因组全序列分析探讨浙江省1998-2009年H3N2亚型流感病毒的基因变异与流感流行的关系.方法 选择浙江省1998-2009年流感流行期间分离到的H3N2亚型流感病毒流行代表株19株,采用RT-PCR方法扩增其8个基因片段后进行序列测定,并与近十余年间使用的疫苗株序列进行比较.结果 H3N2亚型流感流行株HA和NA基因的氨基酸变异率最高,分别为13.98%和10.00% 其他6个内部基因所表达的蛋白中,除NP、M2和NS1（氨基酸变异率分别为6.43%、6.19%和3.48%）外,其他蛋白的氨基酸变异均＜3.00% 在H3N2亚型流感存在较大范围流行的年份,除HA和NA基因外,流感病毒其他6个内部基因也会发生不同程度的变异.此外,在以往一些年份中,WHO推荐的H3N2流感病毒疫苗株与浙江省同期流行株之间,在许多基因上存在着滞后现象.结论 在加强对H3N2亚型流感毒株表面HA与NA基因监测的同时,也应注意到其他内部基因的变异,为新一轮流感流行株的出现提供重要的预警信息.Objective To analyze the relationship between influenza epidemic and genetic characteristic on the whole genome of influenza virus subtype A/H3N2 strains isolated in Zhejiang province during 1998 to 2009. Methods All of the eight genes from the 19 Zhejiang influenza virus isolates, circulated during 1998 to 2009, were amplified by RT-PCR and sequenced. The obtained sequences were aligned and analyzed with the vaccine strains being used in the last 10 years.Results The highest mutation happened within HA and NA genes and the amino acid divergent ratios were 13.98% and 10.00%. Amongst the six internal proteins, the amino acid divergent ratios of NP, M2 and NS1 were 6.43%, 6.19% and 3.48% respectively, and the others were lower than 3%.Other than the HA and NA genes, mutations were also observed on six internal genes of the strains isolated in those years when the influenza virus subtype A/H3N2 was widely circulating.Additionally, there had been an obvious genetic lag between vaccine strains recommended by WHO and the contemporary Zhejiang epidemic strains for many years. Conclusion Besides on HA and NA genes, surveillance programs should also be covered mutations regarding the internal genes of influenza virus subtype A/H3N2 strains, in order to provide important information for forecasting and warning of a new round of influenza epidemic.

关 键 词：流感病毒 H3N2亚型 全序列 

分 类 号：R373[医药卫生—病原生物学]