纳秒级脉冲电场诱导肿瘤细胞凋亡的窗口效应  

Window Effect of Tumor Cell Apoptosis Induced by Nanopulses

作　　者：文燕青[1] 姚陈果[2] 唐均英[1] 郭飞[2] 李成祥[2] 米彦[2] 孙才新[2] 

机构地区：[1]重庆医科大学附属第一医院,重庆400016 [2]重庆大学输配电装备及系统安全与新技术国家重点实验室,重庆400030

出　　处：《高电压技术》2010年第11期2797-2802,共6页High Voltage Engineering

基　　金：国家自然科学基金重点项目(50637020)~~

摘　　要：ns级脉冲电场诱导肿瘤细胞凋亡用于肿瘤病变的治疗,展现出令人振奋的临床应用前景。由于ns级脉冲电场诱导的凋亡率高低与场强-脉宽等参数存在一定的选择性,使得目前脉冲参数的选择存在较大的盲目性。为寻求诱导肿瘤细胞有效凋亡的ns级脉冲电场参数,将不同参数组合(电压幅值9 kV,脉宽分别为1、50、100与200 ns,脉冲个数分别为10、30、60与80,频率1 Hz)的ns级脉冲电场作用于SKOV3细胞。利用AnnexinV/PI双染法检测细胞早期凋亡率和坏死率,DNA ladder凝胶电泳实验检测细胞晚期凋亡情况,AnnexinV/PI双染法以及DNA ladder凝胶电泳实验表明:细胞的早期凋亡率同施加的脉冲电场参数存在窗口效应,且脉冲参数为100 ns、30个脉冲时可得到最大的凋亡率与坏死率差值。Tumor cells apoptosis induced by nanopulses has a great potential in clinical tumor treatment.It is difficult to find appropriate parameters of nanopulses because of the selectivity between apoptosis rate induced by nanopulses and parameters of nanopulses such as field intensity and pulse width etc.To obtain the appropriate parameters of nanopulses which can induce effective tumor cell apoptosis,different parameters of nanopulses（voltage amplitude is 9 kV;pulse duration are 1,50,100 and 200 ns respectively;pulse number are 10,30,60 and 80 respectively;repetition frequency is 1 Hz） were tested on SKOV3 cells.AnnexinV/PI was used to test early stage apoptosis rate and necrosis rate of tumor cells,while DNA ladder gel electrophoresis was used to detect late stage apoptosis of tumor cells.AnnexinV/PI and DNA ladder gel electrophoresis experiments indicate that,window effects of nanopulses on cell early stage apoptosis exist,and nanopulses with the parameters of 100 ns duration and 30 pulses can achieve the highest apoptosis and necrosis rate difference.

关 键 词：ns级脉冲电场 肿瘤细胞 凋亡率 SKOV3细胞 窗口效应 凋亡率与坏死率差值 

分 类 号：Q27[生物学—细胞生物学]