用SSR分子标记检测杂交油菜秦优7号的种子纯度  

Seed Purity Test of Hybrid Rapeseed Qinyou No.7 with SSR Molecular Markers

作　　者：穆建新[1,2] 李殿荣[2] 郭蔼光[1] 王灏[2] 赵晓萍[2] 

机构地区：[1]西北农林科技大学,陕西杨凌712100 [2]陕西省杂交油菜研究中心,陕西大荔715105

出　　处：《西北农业学报》2010年第11期64-68,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西省自然科学研究计划项目(2004C108)

摘　　要：杂交油菜中的主要杂株类型是母本不育株和父本恢复株。通过对100对SSR引物筛选,获得1对可将杂交油菜秦优7号及其父母本区分开来的SSR引物SA82,扩增产物杂交种表现为父母本的互补带型,在30 g/L的琼脂糖凝胶上显带清晰稳定。通过简化油菜DNA提取方法、优化反应体系、重复使用琼脂糖凝胶等,建立了适用于杂交油菜秦优7号种子纯度检测的SSR技术系统。经对大量油菜杂交种个体进行验证,证明该方法具有很好的重复性、准确性和可靠性,同时具有快速、经济的特点,可用于杂交油菜种子纯度的实际检测。The female fertile plants and male restore plants are the two types of the abnormal plants in hybrid rapeseed seed production.One SSR primer named as SA82 was selected out from 100 pairs of primers,which can distinguish hybrid rapeseed Qinyou No.7 and its parents.The pattern of Qinyou No.7 amplified with SA82 show complementary pattern,which could be detected clearly and appeared stable on 30 g/L agarose gels.Hybrid rapeseed Qinyou No.7 seed purity test system was established with SSR marker technique by simplifying the DNA extraction method,optimizing the PCR amplifying conditions and repeatedly using agarose gels,etc.The repeatability,accuracy and reliability of this test technique were proved to be good by analyzing a large numbers of individual plants from different sources.The technique has efficient and economical characteristics and could be used in rapeseed seed purity analysis in practice.

关 键 词：SSR 杂交油菜 种子纯度检测 

分 类 号：S565.4[农业科学—作物学]