加替沙星聚乳酸纳米粒的包封率测定  

Determination of Encapsulation Rate of Gatifloxacin-loaded Polylacticacid Nanoparticles

作　　者：梁健健[1] 张蜀[2] 邓红[2] 林华庆[2] 杨光耀[2] 

机构地区：[1]广州市第一人民医院药剂科,广州510180 [2]广东药学院药物研究所/广东省药物新剂型重点实验室,广州510006

出　　处：《中国现代应用药学》2010年第11期1018-1020,共3页Chinese Journal of Modern Applied Pharmacy

基　　金：广东省科技计划项目(2006B19901005)

摘　　要：目的建立加替沙星聚乳酸纳米粒的包封率测定方法。方法采用葡聚糖凝胶柱层析法,以水为洗脱剂,以l mL.min·1的流速洗脱,采用反相高效液相色谱法测定药物含量,计算包封率。色谱柱为C18柱（150 mm×4.6 mm,5μm）,流动相为0.02 mol.L·1枸橼酸溶液（内含0.6%三乙胺）-甲醇（60∶40）,检测波长为290 nm,流速为1.0 mL.min-1,进样量20μL。结果加替沙星在2.051-60.14μg.mL·1内线性关系良好（r=0.999 9）。平均柱回收率为98.8%,RSD=0.71%;测得3批纳米粒的平均包封率为82.1%,RSD为2.2%。结论建立的方法可用于加替沙星聚乳酸纳米粒的包封率测定。OBJECTIVE To establish a method for the detection of encapsulation rate of gatifloxacin in gatifloxacin-polylacticacid nanoparticles.METHODS The analysis was performed on a sephadex G-50 column,using distilled water as eluent,and detected by HPLC with a C18 column（150 mm×4.6 mm,5 μm） using 0.02 mol.L·1 citric acid（0.6% triethylamine）-methanol（60 ∶ 40） as mobile phase under the flow rate of 1.0 mL.min？1.The detection wavelength was 290 nm.RESULTS Gatifloxacin had a good linear range of 2.051？60.14 μg.mL·1（r=0.999 9）.The average column recovery rate was 98.8% with RSD of 0.71%.The average encapsulation rate of 3 batchs nanoparticles was 82.1% with RSD of 2.2%.CONCLUSION The method can be used to detect the encapsulation rate of gatifloxacin in the nanoparticles.

关 键 词：加替沙星 聚乳酸纳米粒 包封率 葡聚糖凝胶柱法 

分 类 号：R943.3[医药卫生—药剂学] R917.101[医药卫生—药学]