阿莫西林与甲基紫的共振瑞利散射及应用  

Resonance Rayleigh Scattering Spectra of Interaction of Amoxicillin with Methyl Violet and Their Analytical Applications

作　　者：江虹[1] 庞向东[1] 周国静[1] 

机构地区：[1]长江师范学院化学化工学院,重庆408100

出　　处：《中国现代应用药学》2010年第11期1021-1024,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：重庆市教委科技基金资助项目(KJ071304);长江师范学院科研资助项目(2008xky39)

摘　　要：目的建立测定痕量阿莫西林的共振瑞利散射法。方法以甲基紫作探针的共振瑞利散射法测定阿莫西林。结果在pH 9.90的Tris-HCl溶液中,阿莫西林和甲基紫相互作用后,共振瑞利散射显著增强,在376 nm处的△IRRS最强。阿莫西林的浓度在2.0×10-8~1.5×10-6 mol.L-1内与△IRRS成正比,检出限（3Sb/S）为0.042μg.mL-1。结论该法简便、快速、灵敏,可用于痕量阿莫西林的测定。OBJECTIVE To develop a new sensitive Resonance Rayleigh scattering（RRS） method for the determination of trace amoxicillin.METHODS RRS spectra method was used for the determination of amoxicillin with methyl violet as probe.RESULTS In pH 9.90 Tris-HCl buffer solution,the RRS intensity of methyl violet was greatly enhanced when amoxicillin was added.And the strongest ·IRRS was located at 376 nm.The intensity of RRS was in proportion to the concentration of amoxicillin in the range of 2.0×10-8-1.5×10-6 mol.L-1 with a detection limit（3Sb/S） of 0.042 μg.mL-1.CONCLUSION The method showed advantage of convenience,rapidity,high sensitivity and was applied in the determination of trace amoxicillin with suitable results.

关 键 词：共振瑞利散射 阿莫西林 甲基紫 

分 类 号：R978.11[医药卫生—药品] R917.102[医药卫生—药学]