数控剪应力微流装置评价血塞通注射液对胶原诱导血小板黏附与聚集的影响  

Influence of Xuesaitong Injection on Collagen Induced Platelet Adhesion and Aggregation Investigated by Shear Stress Controlled Microfluidic Device

作　　者：张毅[1] 林岭[1] 廖福龙[1] 游云[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国药物警戒》2010年第12期715-718,共4页Chinese Journal of Pharmacovigilance

基　　金：国家自然科学基金(30801530);中国中医科学院基本科研自主选题项目(ZZ20090109)

摘　　要：目的实时观察血塞通注射液对I型胶原诱导血小板黏附与聚集的影响。方法利用Bioflux1000控剪应力微流装置,将I型胶原溶液灌注铺被于微流通道。大鼠腹主动脉取血,枸橼酸钠抗凝。全血以Calcein AM荧光标记后,与血塞通注射液孵育10min,然后被灌注于微流通道,计算机实时采集血小板黏附及血栓形成过程的荧光图像。利用Montage软件进行图像数字化分析。结果 10dyn/cm2的流动条件下,I型胶原可以诱导血小板形成稳定的黏附与聚集;血塞通注射液抑制血小板黏附与聚集的半数有效浓度为0.25mg/mL。血塞通注射液抑制血小板聚集的效应随剪应力的增大而减小,二者呈对数关系拟合,Inhibition(%)=108.17-25.613Ln(τ)(R2=0.97)。结论在流动状态下,血塞通注射液可以抑制胶原诱导的血小板黏附与聚集;血流剪应力的大小影响药效;Bioflux1000可以用于活血化瘀药物的评价。Objective To observe the real-time influence of Xuesaitong injection on the collagen I induced platelet adhesion and aggregation.Methods Bioflux1000 microfluidic device was employed in this study for the first time.The observation channels were coated with collagen I.SD rats underwent anesthesia and blood withdrawn from aortic vessels with 3.8% sodium citrate 1：9 anticoagulation.Whole blood with Calcein AM（4μM） fluorescent labeling for 1 hr.Different concentration of Xuesaitong Injection were added to whole blood incubated for 10 minutes in room tempreture.Whole blood was perfused from the wells at different shear stress for 5 minutes.Fluorescent micrographs were captured with the blood under flow using an inverted microscope and CCD Camera.Inhibition rates by medicine were calculate by percent of control.Results Exposure to collagen I mediated stable platelet adhesion and aggregation within two to three minutes under 10dyn/cm2 flow.The IC50 of Xuesaitong injection on inhibiting platelet adhesion and aggregation is 0.25mg/ml under 10dyn/cm2 flow.By curve estimate we found the logarithm relation between shear stress and efficacy（R2=0.97）.Conclusion Xuesaitong injection could inhibit collagen-induced platelet adhesion and aggregation under flow;Shear stress showed impact on the efficacy of medicine;Bioflux1000 could be used to evaluate the efficacy of Chinese medicine.

关 键 词：血塞通注射液 剪应力 血小板 黏附 微流装置 

分 类 号：R285.5[医药卫生—中药学]