多浆旱生植物霸王高频组培再生体系的建立  

Establishment of a high frequency regeneration system for the succulent xerophyte Zygophyllum xanthoxylum

作　　者：冯波[1] 段娇娇[1] 伍国强[1] 王锁民[1] 

机构地区：[1]兰州大学草地农业科技学院,甘肃兰州730020

出　　处：《草业学报》2010年第6期140-146,共7页Acta Prataculturae Sinica

基　　金：863计划(2006AA10Z126);国家自然科学基金(30770347;30671488)资助

摘　　要：本研究以多浆旱生植物霸王为材料,通过对不同激素组合和外植体(子叶、茎、茎节、下胚轴)的研究,建立了一个稳定、高效的霸王组培再生体系。结果表明,霸王子叶是诱导愈伤组织的最佳外植体,茎节是诱导不定芽的最适外植体;最佳愈伤组织诱导培养基是含有0.1 mg/L 6-BA和1.0 mg/L NAA的MS,子叶在此培养基上愈伤组织诱导率为100%,诱导时间为20 d,优于已报道的30 d;霸王茎节不定芽诱导的最适培养基为附加1.5 mg/L6-BA和0.5 mg/L NAA的MS,诱导率高达82.5%,诱导时间为7周,优于已报道的10周;诱导生根的最佳培养基为含1.0 mg/L NAA和0.5 mg/L IBA的1/2 MS,生根率高达86.7%,比已报道的73.3%的生根率提高了13.4%,且在此培养基上13 d开始出现不定根,早于已报道的20 d;本试验从诱导愈伤组织到再生苗成株需(84±4)d。The effect of compounding proportions of different hormones on explants （cotyledon, stem, eustipes, and hypocotyl） were studied in tissue cultures of the succulent xerophyte Zygophyllum xanthoxylum, and a stable, high-efficiency plant regeneration system was established. Cotyledons were the best type of explant for callus induction and eustipes were the best for adventitious bud induction. The best callus inducing medium for Z. xanthoxylum was MS containing 0.1 mg/L 6-BA and 1.0 mg/L NAA, in which the callus induction rate for cotyledons was 100% with an induction time of 20 d, shorter than the previously reported 30 d. The best medium to induce adventitious buds was MS containing 1.5 mg/L 6 BA and 0.5 mg/L NAA, in which shoot differentiation of eustipes reached 82.5% and the induction time wais 7-weeks, shorter than the earlier report of 10- weeks. The best medium for rooting was 1/2 MS containing 1.0 mg/L NAA and 0.5 mg/L IBA, in which the frequency of rooting reached 86. 7%, 13. 4% greater than the previous result of 73. 3%. In this medium, adventitious roots began to appear in 13 d （earlier than the reported 20 d） and the transition from callus to regeneration plant was completed in （84±4） d. regeneration

关 键 词：霸王 外植体 激素组合 愈伤组织 植株再生 

分 类 号：S184[农业科学—农业基础科学]