硝化细菌单菌分离方式的探讨  

Discussion about Nitrifying Bacteria in Single-colony Isolation Mode

作　　者：王小菊[1] 赵光华 王震[3] 邢传宏[3] 闫爽[1] 宋安东[1] 陈红歌[1] 

机构地区：[1]河南农业大学生命科学学院,河南郑州450002 [2]河南省农科院质量检测中心,河南郑州450002 [3]郑州大学水环学院,河南郑州450001

出　　处：《环境科技》2010年第6期8-10,共3页Environmental Science and Technology

基　　金：国家科技重大专项"水体污染控制与治理"资助(2009ZX07210-002)

摘　　要：通过2种单菌分离方式即直接分离和先富集再分离,利用选择性培养基获得转化NO2-→NO3-的硝化细菌,根据化学法和离子色谱法检测不同分离方法得到的单菌的硝化速率,结果表明先富集再分离所得菌株的硝化速率显著高于直接分离的菌株。This article described two modes of separation of nitrifying bacteria： direct isolation and isolation after enrichment,by using selective media.Different nitrifying bacteria specifically transferring NO2-to NO3-were obtained and their nitrification rates were detected by chemical method and ion chromatography method.The results showed that the nitrification rates of bacteria obtained through separation after enrichment were significantly higher than that of the strains isolated directly.

关 键 词：硝化细菌 单菌分离 亚硝酸盐 

分 类 号：X7[环境科学与工程—环境工程]