高致病性猪蓝耳病与经典猪蓝耳病二重RT-PCR检测方法的建立及应用  

Establishment And Apply of duplex RT-PCR Assay for Detecting Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus And Porcine Reproductive and Resperatory Syndrome Virus

作　　者：杨泽林[1] 曾政[1] 冉智光[1] 米自由 熊仲良[1] 

机构地区：[1]重庆市动物疫病预防控制中心,重庆401120

出　　处：《畜牧市场》2010年第12期10-12,共3页stockbreeding market

摘　　要：高致病性猪蓝耳病病毒(HP-PRRSV)(JX-EF112445)核苷酸序列与经典猪蓝耳病病毒(LP-PRRSV)(U87392)核苷酸序列,设计两对特异性引物,建立了Hp-PRRSV和LP-PRRSV二重RT-PCR快速检测体系。应用此体系成功对1498份组织/血清进行了准确检测。同时,分别与商品化的HP-PRRSV RT-PCR检测试剂盒和LP-PRRSV检测试剂盒进行比对试验,结果吻合率达100%,该方法特异性好、敏感性高、克服了对两种病原进行分别检测所带来的耗时、耗试剂、增加污染几率等风险,为蓝耳病的快速诊断和流行病学调查奠定了基础。Based on the mutation characteristic of genome sequence of highly pathogenic porcine reproductive and respiratory syndrome virus （HP- PRRSV） ,and low pathogenic porcine reproductive and respiratory syndrome virus （LP-PRRSV） , two pair primer s were designed , a rapid duplex RT-PCR method for detecting HP-PRRSVand LP-PRRSV were developed. 1498 samples were detected with the eatablished duplex RT-PCR assay.Compared with commercial kits, the results indicated sensitivity high and specificity good. And the methods avoid the risk of time, reagent consuming , the pollution.the assay laid solid foundation for PRRSV detection and epidemiology.

关 键 词：高致病性猪蓝耳病 猪蓝耳病 二重RT-PCR 

分 类 号：S858.23[农业科学—临床兽医学]