多(二磷酸腺苷-核糖)水解酶抑制剂单宁酸对糖尿病大鼠肾脏病变的影响及其机制  

Effect of poly(ADP-ribose)glycohydrolase inhibitor gallotannin on renal lesion in experimental diabetic rats and its mechanism

作　　者：曲萌[1,2] 赵丽艳[3] 李才[1] 李相军[1] 孙波[1] 魏海峰[1] 

机构地区：[1]吉林大学药学院实验药理与毒理学教研室,吉林长春130021 [2]北华大学医学检验学院分子生物学教研室,吉林吉林132001 [3]吉林大学第二医院检验科,吉林长春130041

出　　处：《吉林大学学报（医学版）》2010年第6期1026-1029,F0002,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30570855)

摘　　要：目的:研究多(二磷酸腺苷-核糖)水解酶(PARG)抑制剂单宁酸(GLTN)对糖尿病大鼠肾脏病变的影响并探讨其可能的机制。方法:将实验动物随机分为正常对照组(NC组)、糖尿病组(DM组)、糖尿病3-氨基苯甲酰胺(3-AB)处理组(CDT组)、糖尿病GLTN低剂量(20mg·kg-1·d-1)处理组(LDT组)和糖尿病GLTN高剂量(30mg·kg-1·d-1)处理组(HDT组),12周后检查各组动物血糖、血肌酐、尿素氮、尿蛋白排泄率水平及肾脏病变情况,免疫组织化学法检测各组大鼠肾组织PARP阳性信号表达。结果:与DM组比较,LDT和HDT组血糖水平明显降低(P<0.01),血肌酐、尿素氮水平和尿蛋白排泄率也明显降低(P<0.01,P<0.05,P<0.01),LDT和HDT组糖尿病大鼠的肾脏病变程度减轻,肾组织PARP蛋白表达降低(P<0.05)。但LDT和HDT组之间上述肾功能参数比较差异无显著性(P>0.05)。结论:GLTN可降低糖尿病大鼠的血糖水平,抑制肾组织PARP表达,改善肾脏功能和形态异常。Objective To investigate the effect of poly（ADP-ribose）glycohydrolase（PARG）inhibitor gallotannin on renal lesion in STZ-induced diabetic rats and its mechanism.Methods Wistar rats were randomly divided into five groups：normal control （NC）group,STZ-induced diabetic （DM）group,diabetic rats treated with 3-aminobenzamide（CDT）group,diabetic rats treated with gallotannin 20and 30mg·kg-1·d-1 （LDT and HDT）groups.The levels of blood glucose,serum creatinine,blood urea nitrogen,urinary protein excretion were detected after treatment for 12weeks.The renal morphology was observed and the expression of poly（ADP-ribose） polymerase（PARP）was tested by immunohistochemical method.Results Compared with DM group,the levels of blood glucose,serum creatinine,blood urea nitrogen and urinary protein excretion were significantly decreased in both LDT and HDT groups（P0.05or P0.01）.The renal lesion and the signal of positive expressing PARP in renal tissues were markedly reduced in diabetic rats treated with GLTN.But there were no significantdifferencesofthe indexes mentioned above between LDT and HDT groups（P0.05）.Conclusion Gallotannin can lower the level of blood glucose,inhibit the renal PARP expression,and ameliorate the renal functional and morphological abnormalities in diabetic rats.

关 键 词：单宁酸 多(二磷酸腺苷-核糖)水解酶 多(二磷酸腺苷-核糖)多聚酶 糖尿病肾病 

分 类 号：R587.1[医药卫生—内分泌]