人用甲型H1N1流感疫苗两种免疫方法动物免疫效果比较  

Comparsion of immunological efficacy of two methods of H1N1 influenza vaccine

作　　者：惠琦[1,2] 付博[1] 范宇红[1] 官淑红[1] 王平[1] 刘辉[1] 张楠[1] 姜崴[1] 

机构地区：[1]长春生物制品研究所质量保证部,吉林长春130062 [2]吉林大学人兽共患病研究所,吉林长春130062

出　　处：《吉林大学学报（医学版）》2010年第6期1052-1054,共3页Journal of Jilin University：Medicine Edition

基　　金：国家科技攻关项目资助课题(2008BAZ54B03)

摘　　要：目的:比较甲型H1N1流感疫苗0d一针免疫方法与0、21d二针免疫方法的免疫效果,同时观察季节性流感疫苗免疫后是否对甲型H1N1流感病毒具有交叉保护性。方法:用甲型H1N1流感疫苗分别按0d一针及0、21d二针2种免疫方法免疫BALB/c小鼠,首针免疫后14、21、28、35及42d眼眶采血分离血清,用血凝抑制(HI)法测定小鼠血清中甲型H1N1特异性抗体滴度,比较2种免疫方法的免疫效果。用季节性流感疫苗免疫小鼠,14、28d后采血分离血清,用甲型H1N1流感病毒特异性抗原采用HI法测定季节性流感疫苗抗血清是否能与甲型H1N1流感病毒特异性抗原进行非特异性结合。结果:二针免疫的方法在第21天加强免疫一次后,第28、35及42天时抗体滴度明显高于一针免疫方法的抗体滴度,且第42天时的抗体滴度约为一针免疫方法抗体滴度的4倍。用甲型H1N1流感病毒特异性抗原采用HI法测定季节性流感疫苗抗血清,未测出甲型H1N1交叉抗体。结论:0、21d二针免疫的方法在第28天后抗体滴度仍保持较高的水平,明显高于0d一针免疫的方法。季节性流感疫苗对甲型H1N1流感病毒无交叉免疫保护性。Objective To compare of immunological efficacy of H1N1influenza vaccine 0done-dose method and 0, 21d,two-dose method,and observe whether seasonal influenza vaccine has cross protection for H1N1influenza virus.Methods H1N1influenza vaccine with two immunological methods was used to inoculate Balb/c mice.The first,the Balb/c mice were inoculated in 0d.The second,the Balb/c mice were inoculated in 0and 21d.The serum from orbital in 14,21,28,35,42dafter the first-dose were separated.HI was performed to determine the H1N1-specific serum antibody titers to compare the immune effect of the two immunological methods.Then the Balb/c mice were inoculated with seasonal influenza vaccine.The serum from orbital in 14and 28dwere separated. HI was performed to determine whether the anti-serum of seasonal influenza vaccine could non-specificly bind with H1N1influenza virus-specific antigens.Results The 28,35and 42dantibody titers of the two-dose method were significantly higher than those of one-dose methods after the second immunity at the 21th day.And the 42d antibody titers of the two-dose methods were 4times of that of one-dose methods.HI was performed to determine the anti-serum of seasonal influenza vaccine,there were is no H1N1influenza virus-specific antibodis in it. Conclusion The antibody titers of 0and 21dtwo-dose method still maintain the high level at 28th day,and are obviously higher than those of one-dose methods.There is no cross-protective immunity betweenseasonalinfluenzavaccine and influenza A H1N1influenza virus.

关 键 词：甲型H1N1流感疫苗 季节性流感疫苗 特异性抗体 交叉免疫保护性 

分 类 号：R186[医药卫生—流行病学]