HPLC测定更年安胶囊中大黄素的含量  

Determination of emodin in Gengnianan capsule by HPLC

作　　者：侯宜[1] 尹建元[2] 李宏斌[3] 李艳艳[2] 张莹[2] 李巍[2] 孟庆雯[4] 孟勤[5] 

机构地区：[1]吉林大学药学院组织工程教研室,吉林长春130021 [2]吉林大学药学院天然药物化学教研室,吉林长春130021 [3]吉林大学化学学院新民化学中心,吉林长春130021 [4]东北师范大学附属中学,吉林长春130021 [5]吉林大学药学院药学实验中心,吉林长春130021

出　　处：《吉林大学学报（医学版）》2010年第6期1186-1188,共3页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科研基金资助课题(200905140)

摘　　要：目的:建立更年安胶囊中大黄素含量的高效液相色谱法(HPLC)测定方法,为工业生产的质量控制提供依据。方法:采用HPLC测定更年安胶囊中大黄素含量,具体条件为:Shimpack VP-ODSC18(150mm×4.6mm,5μm)色谱柱,甲醇-0.1%磷酸水溶液(77∶23)为流动相;检测波长为254nm,柱温为40℃。结果:大黄素在0.04～0.32μg范围内有良好线性关系,回归方程为Y=1060+2513102X,r=0.9991,平均回收率98.6%,RSD为1.88%(n=6);以精密度实验、稳定性实验和重复性实验考察的RSD分别为0.86%、2.33%和1.88%。结论:HPLC测定更年安胶囊中大黄素含量方法可靠,简单可行,可为控制更年安胶囊的内在质量提供科学依据。Objective To establish the high performane liqnid chromatography（HPLC）for determining emodin in Gengnianan capsule,and provide the basis for the quality control of industrial production.Methods The HPLC was developed to determine the emodin in Gengnianan capsule.The developed conditions of HPLC were as follows： the Shimpack VP-ODS C18 （150mm×4.6mm,5μm）,methanol-0.1% phosphoric acid solution（77：23）as mobile phase,the detection wavelength of 254nm,and the column temperature of 40℃.Results The linear range of emodin was 0.04-0.32μg,the regression equation was Y=1060＋2513102X,r=0.9991.The average recovery was 98.6%,and the RSD was 1.88% （n=6）.The RSD in accuracy,stability and repeatability test were 0.86%,2.33%and 1.88%,respectively.Conclusion This method is accurate,simple and reliable,which provide the scientific basis for the quality control of Gengnianan capsule.

关 键 词：更年安胶囊 大黄素 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]