黄芪中主要活性成分提取工艺的研究  

Research of the Extraction of Main Components of Astragalus in Compound Preparation

作　　者：昝日增[1] 胡万杨[2] 余跃[2] 宋霄宏[2] 

机构地区：[1]武警浙江省总队杭州医院,浙江杭州310051 [2]浙江中医药大学药学院,浙江杭州310053

出　　处：《中华中医药学刊》2010年第12期2570-2573,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省科技厅资助项目(2007C33048)

摘　　要：目的:探讨从黄芪中同时获取黄芪甲苷和多糖的方法,为确立结肠靶向制剂的最佳提取工艺提供依据。方法:以黄芪甲苷为醇提部分含量测定指标,用超高效液相色谱-蒸发光散射检测器进行测定;以黄芪多糖为水提部分含量测定指标,经苯酚-硫酸法显色,用紫外可见分光光度法进行测定,通过综合评分优选最佳提取工艺。结果:黄芪以70%乙醇,8倍量,提取2次,每次1.5h,提取黄芪甲苷,药渣再用6倍水,提取3次,每次2h,提取黄芪多糖。结论:选出的提取工艺稳定、合理、可行。Objective： To research the extraction to obtain both Astragaloside and polysaccharide so as to provide the basis for the best extraction technology of colon targeted drugs delivery.Methods： In the alcohol extraction part,Astragaloside Ⅳ was determined by UPLC-ELSD as the content mensuration index;in the aqueous part,APS was determined by UV-visible spectrophotometer through Phenol-sulfuric acid method as the content mensuration index,then mark the result to optimise the best extraction of the main component of Astragalus.Results： The optimum extracting condition to extract astragaloside was adding 70 % alcohol with 8 folds to reflux and extract for 2 times,1.5 hours each time.Dregs then was extracted by adding 6 times amount of water to reflux and extract for 3 times,2 hours each time to extract APS.Conclusions： The optimized process is stable and highly efficient.

关 键 词：超高效液相色谱-蒸发光散射检测 苯酚硫酸法 黄芪皂苷 黄芪多糖 提取工艺 

分 类 号：R285.5[医药卫生—中药学]