重组人促红细胞生成素鼻腔给药对大鼠慢性脑缺血的保护作用  

Protective effects of intranasal erythropoietin on chronic cerebral ischemia in rats

作　　者：叶夷露[1] 张琦[1] 季华[1] 张建亭[1] 黄春燕[1,2] 俞月萍[1] 饶艳[1] 

机构地区：[1]浙江医学高等专科学校 [2]温州医学院

出　　处：《中国临床药理学与治疗学》2010年第9期997-1001,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：浙江省卫生厅科研项目(2007A028;2008B034)

摘　　要：目的:观察重组人促红细胞生成素(rhE-PO)鼻腔给药对大鼠慢性缺血性脑损伤的保护作用。方法:以大脑中动脉栓塞诱导大鼠局灶性脑缺血,手术当日术前30 min和术后2 h各给药一次,第2天起隔天一次,持续到术后14 d。缺血后1、3、7、14、21、283、5 d进行神经症状评分和斜板试验,缺血后35 d计算脑梗死体积和缺血周边区神经元密度。结果:rhEPO鼻腔给药(24、48 U)、依达拉奉和rhEPO腹腔给药(5000 U/kg)能改善缺血后神经症状评分,提高斜板角度,降低脑梗死体积,增加缺血侧周边区存活神经元密度。结论:rhEPO鼻腔给药对大鼠慢性缺血性脑损伤具有保护作用,其有效剂量范围较腹腔给药低。AIM：To investigate the protective effects of recombinant human erythropoietin（rhEPO） administrated intranasally on chronic cerebral ischemia in rats. METHODS：Focal cerebral ischemia in rats were induced by middle cerebral artery occlusion（MCAO）.Drugs were injected intranasally or intraperitoneally 30 min before and 2 h after operation.From the second day,they were administrated qod for 2 weeks.In addition to neurological deficit and inclined board test during 35 d,we detected infarct volume and the survival neuron density in the ischemic brain tissue 35 d after operation.RESULTS：rhEPO（24,48 U） intranasally,edaravone and rhEPO i.p.（5000 U/kg） significantly attenuated neurological deficit and infarct volume,increased the angle in the board test and the survival neuron density in the border of ischemia.CONCLUSION：rhEPO intranasally demonstrates protective effect on chronic cerebral ischemia in rats.Especially the effective dosage of rhEPO intranasally far is smaller than intraperitoneally.

关 键 词：重组人促红细胞生成素 鼻腔给药 脑缺血 大鼠 

分 类 号：R965.2[医药卫生—药理学]