草酸铂处理人结肠癌细胞蛋白质组分析  

Proteome analysis of human colon cancer cell line treated by oxaliplatin

作　　者：陈家诚[1] 吴静[2] 钟亮玉[2] 郭伟[3] 陈远光[1] 

机构地区：[1]广州医学院第一附属医院胃肠外科,510120 [2]广州医学院第一附属医院消化内科,510120 [3]广州医学院第一附属医院急诊科,510120

出　　处：《中华生物医学工程杂志》2010年第3期187-190,共4页Chinese Journal of Biomedical Engineering

基　　金：基金项目：广东省卫生厅项目（A2006283）

摘　　要：目的分析草酸铂处理后人结肠癌Lovo细胞系的差异表达蛋白，从蛋白质组学角度探讨草酸铂的作用机制。方法Lovo细胞系按完全随机法分2组，即实验组（含草酸铂培养液培养）和对照组（无草酸铂培养液培养）。培养后收集细胞依次进行二维电泳分离并选择差异点，采用质谱分析鉴定差异蛋白。结果两组双向电泳图谱分辨率高、重复性好，匹配率分别为80．16％（812／1013）和81．19％（829／1021）。获取重复差异点22个，质谱分析其中10个，成功鉴定出与结肠癌相关6种蛋白：B2微球蛋向、剪接因子SFRS3、尿嘧啶DNA糖基化酶表达上调；60s核糖体蛋向P2、甘油醛3磷酸脱氢酶、鸟苷酸结合蛋白B2多肽样1表达下调。结论草酸铂作用人结肠癌Lovo细胞系后有多种差异蛋白表达，其与免疫增强、pre—mRNA转录修饰、DNA损伤修复、调控蛋白翻译及翻译后加丁、能量代谢及信号传导通路等有关。Objective To analyze differential expression proteins of human colon cancer Lovo cell line treated by oxaliplatin, and to explore the antitumor mechanism of oxaliplatin in the view of proteomies perspective. Methods The Lovo cells were randomly divided into two groups, including the experimental group （cultured in oxaliplatin-containing solution） and the control group （cultured in oxaliplatin- free solution）. After culture, the both groups were processed by two-dimensional gel electrophoresis to select disparates, and differential proteins were identified by mass spectrometry analyses. Results High resolution and good reproducibility were observed on electrophoretograms of both groups, with matching rates at 80.16% （812/1013） and 81.19% （829/1021） respectively. Twenty- two repeated disparate points were ohtained, ten of them were selected for mass speetrometry analyses. Six colorectal cancer related proteins were successfully identified, including 3 up-regulated proteins （β-2 microglobulin, splicing factor SFRS3 and uracil-DNA glycosylase） and 3 down-regulated proteins （60 S acidic ribosomal protein, glyceraldehyde- 3-phosphate dehydrogenase and guanine nucleotide binding protein [3 polypeptide 2-like 1 ）. Conclusion Multiple differential protein expressions are identified in human colon cancer Lovo cell line after oxaliplatin treatment, and the proteins are related to immune enhancement, pre-mRNA transcription modification, DNA damage repair, protein translation regulation, post- translation processing, metaholism of energy and signaling transduction pathway.

关 键 词：草酸铂 结直肠肿瘤 质谱分析法 电泳 凝胶 双向 蛋白质组学 

分 类 号：R96[医药卫生—药理学] R735.35[医药卫生—药学]