航天特定环境下抑郁症模型大鼠血清、海马及延髓中单胺神经递质含量变化  

Changes of monoamine neurotransmitters in serum, hippocampus and medulla obiongata of experimental depression .rat models under simulated space-specific environment

作　　者：刘军莲[1] 李勇枝[1] 白延强[1] 米涛[1] 赵爽[1] 辛冰牧[1] 

机构地区：[1]中国航天员科研训练中心,北京100094

出　　处：《中华生物医学工程杂志》2010年第4期281-283,共3页Chinese Journal of Biomedical Engineering

基　　金：基金项目：国家自然科学基金（30772694）

摘　　要：目的研究航天特定环境下抑郁症模型大鼠血清、海马及延髓中单胺神经递质的变化。方法选择旷场实验行为学评分相近的30只大鼠，按完全随机法分为对照组和模型组，每组15只。模型组采用经典抑郁症模型，结合航天特定环境，建立大鼠抑郁症模型，对照组不做任何处理。观察航天特定环境下抑郁症模型大鼠行为学变化，测定血清、海马、延髓组织中5－羟色胺（5-HT）、多巴胺（DA）及去甲肾上腺素（NE）含量的变化。结果与对照组比较，造模后模型组大鼠旷场实验中的穿行格数和直立次数减少，理毛次数、中央格停留时间增加（均P〈0．05）；模型组大鼠血清中5－HT、DA及NE含量均高于对照组[（334．23±110．45）μg/L比（248．76±51．83）μg/L，（109．19±26．86）μg/L比（88．42±12．63）μg/L，（102．46±39，31）μg/L比（74．88±12．04）μg/L，均P〈0．05）]，而海马组织中NE含量低于对照组[（9．99±8．02）μg/L比（25．41±5．68）μg/L，P〈0．05]。结论航天抑郁症的产生可能与血清中5－HT、DA降低无关，而与海马组织中的NE降低有关。Objective To explore the changes of monoamine neurotransmitters in serum, hippocampus and medulla oblongata of experimental depression rats under simulated space-specific environment. Methods Thirty rats with similar behavior scores at open field test were randomized into model group and control group （n=15 each）. Classical pattern of depression combined with simulated space- specific environment was used to establish the rat models of depression in the model group, whereas the rats in the control group received no treatment. Under simulated space-specific environment, the changes of behavior were observed and the changes of neurotransmitters including 5 - hydroxytryptamine （5 - HT） , dopamine （DA） and norepinephrine （NE） in serum, hippoeampus and medulla oblongata were measured. Results Compared with controls, the depressed rats walked through fewer squares, experienced less upright actions, more grooming and time at the central square （all P〈 0.05）. In the model rats, the serum contents of 5-HT [ （334.23±110.45）μg/L vs （248.76±51.83）μg/L, DA [（109.19±26.86）μg/L vs （88.42±12.63） pLg/L] and NE [ （ 102.46±39.31 ）μg/L vs （74.88±12.04） ] were significantly elevated （all P〈0.05）, and NE [ （9.99±8.02） μg/L vs （25.41±5.68 ） μg/L, P〈0.05 ] in hippocampus was reduced, as compared with controls. Conclusion The major cause of space environment-related depression may be associated with the reduced level of NE in hippoeampus and not associated with the reduced levels of 5-HT and DA in serum.

关 键 词：航天医学 抑郁 5-羟色胺 多巴胺 去甲肾上腺素 

分 类 号：R285.5[医药卫生—中药学]