骨碎补总黄酮对大鼠骨髓间充质干细胞成骨分化过程中TGF-β1、BMP-2分泌的影响  

Effect of drynaria total flavonoid on TGF-β1 and BMP-2 secretion in the process of rats bone mesenchymal stem cells osteogenic differentiation

作　　者：舒晓春[1] 朱丹华[1] 鲁红云[1] 庞天娇[1] 孙辽[1] 叶礼红[1] 沈晶[1] 谢丹红[1] 

机构地区：[1]中山大学附属第五医院内分泌科,珠海519000

出　　处：《中华内分泌代谢杂志》2010年第11期990-992,共3页Chinese Journal of Endocrinology and Metabolism

基　　金：基金项目：国家中医药管弹局科研基金（04-05ZP51）;广东省中医药管理局科研基金（2007263）

摘　　要：将大鼠分为空白对照组、经典组和骨碎补组,采用全骨髓贴壁法分离纯化大鼠骨髓间充质干细胞（BMSCs）,检测BMSCs成骨分化过程碱性磷酸酶活性、矿化结节、转化生长因子-β1（TGF-β1）和骨形态发生蛋白2（BMP-2）的分泌.结果显示,骨碎补总黄酮促进成骨分化同时增加TGF-β1、BMP-2分泌（均P＜0.05）.骨碎补总黄酮可能通过上调二者的表达促进成骨,对骨质疏松症可能有积极的治疗意义.The rats were assigned to blank control group, classical induction group, and drynaria total flavonoid group. Whole bone marrow culture method was applied to isolate and purify rats bone mesenchymal stem cells （BMSCs）. Akaline phosphatase activity, calcium nodes, TGF-β1 and BMP-2 secretion in the process of bone mesenchymal stem cells osteogenic differentiation were detected. The results showed that compared to the blank group and classical group, drynaria total flavonoid promoted osteogenic differentiation accompanied with increased TGF-β1 and BMP-2 secretion （all P〈0. 05）. Drynaria total flavonoid may promote osteogenic differentiation of BMSCs via upregulating TGF-β1 and BMP-2 expressions, and play an active role in the treatment of osteoporosis.

关 键 词：骨髓问充质干细胞 骨质疏松症 成骨分化 

分 类 号：R686[医药卫生—骨科学]