伸长细胞移植对大鼠脊髓损伤后运动功能及运动诱发电位的影响  

Effects of tanycytes transplantation on the motor function score and rubrospinal motor evoked potentials of adult rats after spinal cord completely transected

作　　者：徐晓雪[1] 邵雪梅[2] 虞芬[3] 刘丽敏[3] 张茂先[2] 高秀来[4] 

机构地区：[1]首都医科大学医学实验与测试中心,北京100069 [2]首都医科大学医学科技处,北京100069 [3]首都医科大学生理教研室,北京100059 [4]首都医科大学组织与胚胎学教研室,北京100059

出　　处：《中国应用生理学杂志》2010年第4期433-435,526,共4页Chinese Journal of Applied Physiology

基　　金：北京市教委基金资助项目(00KJ104)

摘　　要：目的:研究伸长细胞是否可以促进成年大鼠脊髓损伤后传导束再生。方法:采用Wistar大鼠脊髓T8全横断模型,移植传代培养的伸长细胞,以未移植脊髓损伤组为对照,观察两组损伤后第12周末BBB评分,损伤平面以下红核-脊髓运动诱发电位,和横断部位组织学染色结果。结果:第12周末伸长细胞移植组红核脊髓运动诱发电位总峰值显著高于对照组(MD=133.2μV,P<0.01),峰潜伏期较对照组缩短(MD=0.061ms,P=0.040);第12周末伸长细胞移植组BBB评分显著高于对照组(MD=5.0000,P<0.01);第12周末脊髓横断部位HE染色显示伸长细胞移植组脊髓损伤处结构较完整。结论:伸长细胞移植可以促进大鼠脊髓损伤后神经传导的恢复。Objective：To determine whether tanycytes be able to support the regeneration of completely transected spinal cord in adult rats. Methods： Subcultured tanycytes was transplanted into completely T8 transected spinal cord using the untransplanted completely transected rats as control. After transplantation the rubrospinal motor evoked potentials were recorded below the injury level at the end of 12th week,assistant by Basso-Beatie-Bresnahan locomotor rating scale and histology method. Results： At the end of 12th week the total peak amplitude of rubrospinal motor evoked potentials（MD=133.2 μV,P0.01） and BBB locomotor rating scale（MD=5.0000,P0.01） were both significantly improved in cell transplanted group compared with that in the untransplanted control group,while the latency of the first peak was shor-tened（MD=0.061 ms,P=0.040）. HE staining showed more integrity in transected spinal cords in cells transplanted groups. Conclusion： Transplanted tanycytes can support the regeneration of transected spinal cords in rats.

关 键 词：伸长细胞 脊髓损伤 移植 运动诱发电位 红核脊髓束 

分 类 号：R338.2[医药卫生—人体生理学]