Effects of recombinant adeno-associated viruses expressing human vascular endothelial growth factor 165 on spinal cord injury  

作　　者：Chen Zhang Hui Qiang Xiaoqian Dang Kunzheng Wang 

机构地区：[1]First Department of Orthopedics, Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China [2]Department of Orthopedics, Shaanxi Provincial People's Hospital, Third Affiliated Hospital, Medical School of Xi'an Jiaotong University,Xi'an 710068, Shaanxi Province, China

出　　处：《Neural Regeneration Research》2010年第21期1623-1628,共6页中国神经再生研究（英文版）

基　　金：the National Natural Science Foundation of China, No. 30772189

摘　　要：Numerous studies have confirmed that vascular endothelial growth factor （VEGF） improves the function of neural cells following spinal cord injury （SCI）. However, some studies have also verified that VEGF cannot significantly induce the increase in vascular density at or surrounding the lesion, and that VEGF therapy exacerbated secondary damage following SCI. Based on the dual effects of VEGF on SCI, we constructed the recombinant adeno-associated viruses （rAAV）-hVEGF165-IRES-human recombinant green fluorescent protein （hrGFP） （AAV-VEGF） and rAAV-IRES-hrGFP （AAV-GFP）. Our results suggested that rAAV expressed hVEGFles, and a low dose of VEGF relieved increased vascular permeability, improved microcirculation in the local spinal cord, lessened spinal cord edema, and decreased neuronal apoptosis. These results verified that the releasing effects of the rAAV virus vector had protective effects on the spinal cord.Numerous studies have confirmed that vascular endothelial growth factor （VEGF） improves the function of neural cells following spinal cord injury （SCI）. However, some studies have also verified that VEGF cannot significantly induce the increase in vascular density at or surrounding the lesion, and that VEGF therapy exacerbated secondary damage following SCI. Based on the dual effects of VEGF on SCI, we constructed the recombinant adeno-associated viruses （rAAV）-hVEGF165-IRES-human recombinant green fluorescent protein （hrGFP） （AAV-VEGF） and rAAV-IRES-hrGFP （AAV-GFP）. Our results suggested that rAAV expressed hVEGFles, and a low dose of VEGF relieved increased vascular permeability, improved microcirculation in the local spinal cord, lessened spinal cord edema, and decreased neuronal apoptosis. These results verified that the releasing effects of the rAAV virus vector had protective effects on the spinal cord.

关 键 词：spinal cord injury neural regeneration adeno-associated virus vascular endothelia growth factor cell apoptosis 

分 类 号：Q78[生物学—分子生物学] Q782