人发角蛋白材料植入修复皮下脂肪缺损的实验观察  

Experimental study of repairing fat defect with human hair keratin material

作　　者：罗建国[1] 黄犁[2] 姚艳君[3] 肖应庆[5] 傅建民[4] 

机构地区：[1]南方医科大学附属深圳市妇幼保健院整形外科,深圳518028 [2]南方医科大学附属深圳市妇幼保健院病理科,深圳518028 [3]南方医科大学附属深圳市妇幼保健院供应室,深圳518028 [4]南方医科大学附属深圳市妇幼保健院乳腺科,深圳518028 [5]南方医科大学生化教研室

出　　处：《中华整形外科杂志》2010年第6期435-439,共5页Chinese Journal of Plastic Surgery

基　　金：深圳市科技项目（JH200507130980A）

摘　　要：目的 探讨人发角蛋白材料植入皮下脂肪缺损处能否形成新生脂肪组织,旨在为皮下肿物切除术后即时填充修复及矫正陈旧性体表凹陷畸形提供一条新的途径. 方法 采用西藏小型猪3只,在猪脊柱两侧皮下各形成脂肪缺损8处,直径约1.5 cm.将人发角蛋白材料制成直径约1.5 cm球状植入一侧缺损处,另一侧脂肪缺损作为空白对照.采用组织学方法观察术后不同时间人发角蛋白材料降解及新生脂肪组织生成情况. 结果 人发角蛋白材料植入后2周,可见结缔组织及微血管从材料孔隙长入;植入后4周,人发角蛋白基本降解吸收,可见材料碎屑及异物肉芽肿,其周围见成簇的新生脂肪细胞;植入后6周,人发角蛋白材料完全降解,异物肉芽肿也随之消失,代之以新生脂肪组织,其体积与当初植入的人发角蛋白材料体积接近;植入后10周,新生脂肪组织中的纤维成分减少,接近周围正常脂肪组织. 结论 人发角蛋白材料植入皮下脂肪组织缺损处,可在原位形成足够体积的新生脂肪组织,并逐渐塑形,从而在组织结构上接近正常脂肪组织.Abstract：Objective To investigate the feasibility of adipogenesis from human hair keratin （HHK） material, so as to provide a new method for fat defect and depression deformity. Methods 3 Tibet mini-pigs were used. 8 fat defects（ 1. 5 cm in diameter） were made bilaterally on the back. The ballshaped HHK material was implanted to repair the defects at one side. The defects at contralateral side were as controls. The absorption of the HHK material and adiogenesis were studied histologically. Results 2 weeks after implantation, connective tissue and capillary grew into the porous HHK material. 4 weeks after implantation, HHK material was almost totally absorbed, leaving some material debris and foreign body granuloma. Around them, there were clusters of adipocyto. 6 weeks after implantation, the HHK material was totally degraded and the granuloma was disappeared, and then de novo adipose tissue was observed. Its volume was close to the volume of peripheral HHK material that was planted originally. 10 weeks later, the new-formed fat tissue had less fibres and was very similar to the normal fat. Conclusions New adipose tissue can be formed after HKK material implantation. It can also be remodeled to be similar to normal fat.

关 键 词：脂肪缺损 人发角蛋白 脂肪组织 

分 类 号：R318.08[医药卫生—生物医学工程]