转基因衣藻lba及对照藻产氢培养条件的优化  

Optimization of Culture Conditions for Hydrogen Production of Chlamydomonas reinhardtii and its Transgenic Strain lba

作　　者：许丽丽[1] 阎光宇[1] 王全喜[1] 吴双秀[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《中国生物工程杂志》2010年第11期44-49,共6页China Biotechnology

基　　金：上海市部分地方院校能力建设项目(09160500400);上海师范大学原创与前瞻性预研项目(DYL201001);上海师范大学细胞生物学校级重点学科(DZL808)资助项目;上海市教育委员会科研创新项目(08YE66);上海市教育委员会重点学科建设项目(J50401)

摘　　要：通过对莱茵衣藻849及其转基因衣藻lba进行光照强度、细胞浓度和培养基中硫酸盐含量三因素三水平的正交实验,确定了两个藻种的最佳产氢条件,同时对转基因藻和849产氢培养条件下的光合放氧速率和pH进行了检测。实验结果表明,在25℃下,莱茵衣藻849和转基因衣藻lba的最佳产氢条件都为光照强度60μmol/(m2.s),细胞浓度为叶绿素含量12.5μg/ml,培养基中硫酸盐含量0μmol/L。莱茵衣藻849和转基因衣藻lba的最高氢气产量分别达到了349μl/mg chlorophyll和634μl/mg chlorophyll。在产氢条件下,转基因藻lba的净光合放氧速率比849低。结果为利用豆血红蛋白特性通过基因工程手段提高莱茵衣藻产氢量提供基础实验数据。In order to determine the optimal culture conditions for hydrogen production of Chlamydomonas reinhardtii strain 849 and its transgenic alga lba,the orthogonal experiments were designed to test the effects of such three factors as light intensity,cell density and the sulfur content in the culture medium at three levels,meanwhile O2-evolution activity and pH value were detected.The results showed that at 25 ℃,the optimal culture conditions for hydrogen production for both strain 849 and the transgenic alga lba were under 60μmol/（m2·μs）of light intensity,12.5μg/ml of the chlorophyll content as cell density and 0μmol/L of sulfate content in the medium.Under such condition,the maximal hydrogen yield of strain 849 and the transgenic algae lba reached 349μl/mg chl and 634μl/mg chl respectively.The net O2-evolution activity of transgenic alga lba was lower than 849.The results provided basic data for the utilization of leghemoglobin in the improvement of hydrogen production of C.reinhardtii with bioengineering methods in the future.

关 键 词：莱茵衣藻 转基因 豆血红蛋白 lba基因 培养条件优化 

分 类 号：Q785[生物学—分子生物学]