菊花花器性状在F_1代变异及相关联的SRAP分子标记  

Variation of Inflorescence Traits in F_1 Progeny of Chrysanthemum and the Association with SRAP Markers

作　　者：张飞[1,2] 陈发棣[1] 房伟民[1] 陈素梅[1] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]浙江省农业科学院花卉研究开发中心,杭州311202

出　　处：《林业科学》2010年第11期162-167,共6页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(30871724)

摘　　要：花器是菊花（Dendranthema morifolium）观赏特性的重要构成因素,也是菊花品种改良的主要目标性状之一。花器性状比较复杂,主要由花序直径、舌状花数、瓣性和花色等组成。由于栽培菊花的遗传背景复杂,存在高度杂合、自交不亲和、近交衰退等现象,给菊花目标性状改良带来了很大困难。张飞等（2008）研究了花器性状在切花菊品种间的遗传变异情况;菊花部分性状在杂种一代的变异情况也有相关报道（徐文辉等,2000;陈发棣等,2003;蒋甲福等,2003）,但是研究的样本群体都比较小。Variation of inflorescence traits in F1 progeny populations derived from chrysanthemum（Dendranthema morifolium）cultivars ＇Yuhualuoying＇and ＇Aoyunhanxiao＇were investigated and the association between polymorphic SRAP markers and inflorescence traits was analyzed with One-Way ANOVA.The result showed that the 5 inflorescence traits segregated significantly in the F1 population with coefficient of variation ranging from 15.32% to 49.70%,all fitting into a normal distribution.The association analysis between SRAP markers and inflorescence traits suggested that there were 10,8,4,4 and 5 SRAP markers identified to be significantly related with flower diameter,number of ray florets,number of tubular florets,ray floret length and ray floret width,with the cumulative contribution ratio of 35.781%,33.702%,16.175%,15.018% and 20%,respectively.However,the contribution ratio of each single genetic marker was relatively low,varying from 2.815% to 5.882%,which revealed that these markers were polygene with small effect.We anticipate that the proper utilization of these SRAP markers with relatively large contribution ratio,such as Me4Em1-3,Me4Em9-4,Me12Em3-1 and Me12Em16-5,in future gene-cloning studies would effectively improve molecular breeding program for inflorescence traits in chrysanthemum.

关 键 词：菊花 花器性状 SRAP 关联分析 单因素方差分析 

分 类 号：S718.46[农业科学—林学] S682.11