全蝎纯化液对凝血酶诱导血管内皮细胞释放血管性血友病因子和6-酮-前列腺素F1α的影响  

Effects of scorpion purified liquid on the release of von willebrand factor and 6-keto-prostaglandin F1α induced by thrombin in cultured vascular endothelia cells

作　　者：易小民[1] 谭茜[1] 彭延古[1] 徐爱良[1] 黄莺[1] 靳铁飞[1] 

机构地区：[1]湖南中医药大学血管生物学实验室,湖南长沙410007

出　　处：《中国中西医结合急救杂志》2010年第6期334-336,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：基金项目：国家自然科学基金资助项目（30772715）;湖南省教育厅重点资助项目（07A053）;湖南省重点学科实验室资助项目

摘　　要：目的 观察全蝎纯化液对凝血酶诱导血管内皮细胞（VEC）释放血管性血友病因子（vWF）和6-酮-前列腺素F1α（6-keto-PGF1α）的影响,探讨全蝎抗凝作用的机制.方法 体外培养人脐静脉内皮细胞（HUVEC）,选择生长良好的细胞分为空白对照组、凝血酶组及全蝎纯化液高、中、低剂量组,每组10个复孔.全蝎纯化液高、中、低剂量组加入凝血酶10 kU/L后,分别加入全蝎纯化液24、12、6 mg/L;凝血酶组加入凝血酶10 kU/L;空白对照组加入等量RPMI1640培养液.各组均培养12 h后收集上清液,用酶联免疫吸附法（ELISA）检测上清液中vWF、6-keto-PGF1α的含量.结果 与空白对照组比较,凝血酶组细胞培养上清液中vWF含量显著升高[（17.01±0.54）%比（6.05±0.49）%]、6-keto-PGF1α含量显著降低[（20.40±1.18） ng/L比（35.20±1.37） ng/L,均P＜0.01];与凝血酶组比较,全蝎纯化液高、中、低剂量组vWF均显著降低[（7.18±0.44）%、（8.91±0.47）%、（9.81±0.48）%],6-keto-PGF1α含量均显著升高[（30.10±1.50）、（25.50±1.43）、（23.30±1.47） ng/L],差异有统计学意义（均P＜0.01）.结论 全蝎纯化液能抑制凝血酶诱导HUVEC释放vWF,并对抗凝血酶抑制HUVEC释放6-keto-PGF1α,提示全蝎纯化液对凝血酶诱导的VEC损伤具有保护作用.Objective To observe the effect of scorpion purified liquid on the release of von willebrand factor （vWF） and 6-keto-prostaglandin F1α （6-keto-PGF1α） from cultured vascular endothelia cells （VEC） induced by thrombin, and to investigate the mechanism of anticoagulation. Methods The human umbilical vein VEC was cultured in vitro. The well grown VECs were divided into the blank control, thrombin and high, medium, low concentration scorpion purified liquid groups, 10 holes in each group. The thrombin group was given 10 kU/L of thrombin, and different concentrations of scorpion purified liquid （24, 12 and 6 mg/L） were given after administration of thrombin （10 kU/L） in high, medium, low concentration scorpion purified liquid groups. The blank control group was given RPMI1640 culture medium. The VECs were cultured for 12 hours and the supernatant was collected in each group. The concentrations of vWF and 6-keto-PGF1α in the supernatant were measured with enzyme linked immunosorbent assay （ELISA）. Results Compared with the blank control group, the concentration of vWF was significantly increased （（17.01 ± 0. 54）% vs. （6.05 ± 0. 49）%3 and the concentration of 6-keto-PGF1α was markedly decreased in thrombin group [（20.40 ± 1.18） ng/L vs. （35.20!1.37） ng/L, both P±0. 013. Compared with thrombin group, the concentration of vWF was obviously decreased （（7.18 ± 0.44） %, （8.91 ± 0.47） %, （9.81 ± 0.48） %3, the concentration of 6-keto PGF1α was remarkably increased [（30.10±1.50） ng/L, （25.50±1.43） ng/L, （23.30±1.47） ng/L] in high, medium, low concentration scorpion purified liquid groups （all P〈0.01）. Conclusion Scorpion purified liquid can inhibit the release of vWF from HUVEC induced by thrombin and counter the thrombin inhibition on the HUVEC release of 6 keto-PGF1α, suggesting that scorpion protect the injured VEC induced by thrombin.

关 键 词：全蝎 凝血酶 血管内皮细胞 血管性血友病因子 6-酮-前列腺素F1Α 

分 类 号：R285.5[医药卫生—中药学] Q343.6[医药卫生—中医学]