脱氧核糖核酸与双苯甲亚胺相互作用的荧光特性研究  

Fluorescence Characteristics on the Interaction Between Deoxyribonucleic Acid and Bisbenzimide Hoechst 33258

作　　者：何品刚[1] 孙星炎[1] 徐春[1] 刘盛辉[1] 陈奕利 方禹之[1] 

机构地区：[1]华东师范大学化学系,上海200062

出　　处：《分析化学》1999年第4期398-401,共4页Chinese Journal of Analytical Chemistry

基　　金：自然科学基金资助课题;编号为29575197

摘　　要：采用荧光光谱法对脱氧核糖核酸(DNA)与双苯甲亚胺(Hoechst 33258)相互作用的方式及其作用机理进行了研究,证实了Hoechst 33258与DNA的相互作用除嵌入作用外,还存在非特异性的静电作用;Hoechst 33258与DNA主要作用在A-T区域.同时,在磷酸根的存在下可以减弱Hoechst 33258与DNA的非特异性作用.另外,根据荧光峰增强与Hoechst 33258浓度的线性关系,提出了Hoechst 33258的定量测定方法.The interaction mechanism of deoxyribonucleic acid ( DNA) with Hoechst 33258 is studied by fluorescence spectrum in this work. The results show that Hoechst 33258 intercalate between the basic pairs of the double-helical structure of dsDNA, so that its fluorescence increases markedly. As to ssDNA, the fluorescece enhancement is weakened because there is no double-helical structure. The existence of phosphate can weaken the non-specific interaction between Hoechst 33258 and DNA. The main interaction sites of Hoechst 33258 is between base pairs of A and T. The quantity of Hoechst 33258 can be measured by using the linear relationship between the concentration of Hoechst 33258 and thefluorescence Peaks with the existent of amount of DNA. The linear range is 9.0-×10-6～5.0×10-8mol/L, the limit of detection is 1.0×10-8 mol/L(S/N = 3) and the relative standard deviation is2.3%.

关 键 词：荧光光谱法 脱氧核糖核酸 双苯甲亚胺 相互作用 

分 类 号：O625.631[理学—有机化学] Q523.03[理学—化学]