可溶性滤膜富集-分光光度法测定水体中微量蛋白质  

Spectrophotometric Determination of Trace Protein in Water After Preconcentration on an Organic Solvent-Soluble Membrane Filter

作　　者：陈颖[1] 徐辉[1] 王淑英[1] 梁凯[2] 

机构地区：[1]首都师范大学化学系,北京100037 [2]北京工业大学附属中学,北京100022

出　　处：《分析化学》1999年第4期464-467,共4页Chinese Journal of Analytical Chemistry

基　　金：北京市及北京市教委基金资助课题

摘　　要：提出了分光光度法测定蛋白质的新方法.在酸性介质中,蛋白质能和溴连苯三酚红结合成为超分子化合物,用硝化纤维素滤膜富集蛋白质-溴连苯三酚红的复合物,该体系及滤膜可溶解于酸化了的二甲亚砜中形成稳定的有色溶液,其最大吸收波长为425nm.当富集倍数为50时,本方法的检测限为7μg/L,蛋白质在5mL溶剂中的含量为0～350μg时符合朗伯-比尔定律.操作简单,用于样品中蛋白质的测定,结果满意.A new separation method, based on the reaction between protein and bromopyrogallol red (BPR) to form super molecular compound is described. The super molecular compound formed wascollected on a 0.45μm organic solvent-soluble nitrocellulose filter. Then the filter with the complex wasdissolved in a small volume of dimethyl sulfoxide acidified with dilute sulfuric acid. The absorbance of the resulting solution was measured at 425 ran against a reagent blank. The sandell sensitivity was 0. 13μg/cm2 at this wavelength. Beer's law was obeyed in the range of 0-350μg protein in 5 mL of solventwith good reproducibility. Detection limits was better than 7μg/L with a 50-fold enrichment factor. Thecomponents normally present in natural water do not interfere. The proposed method has been applied to the separation and determiantion of protein in urine and water samples. The recovery was above 95.6% .

关 键 词：蛋白质 可溶性滤膜 预富集 分光光度法 测定 

分 类 号：O629.73[理学—有机化学] Q510.3[理学—化学]