高效液相色谱法对化妆品中17种香豆素类化学成分的同时测定  

Simultaneous Determination of 17 Coumarins in Cosmetics by High Performance Liquid Chromatography

作　　者：席海为[1] 马强[1] 王超[1] 白桦[1] 刘茜[1] 王烨[1] 

机构地区：[1]中国检验检疫科学研究院,北京100123

出　　处：《分析测试学报》2010年第12期1168-1172,共5页Journal of Instrumental Analysis

基　　金：质检公益性行业科研专项计划资助项目(200910267)

摘　　要：建立了同时测定化妆品中17种香豆素类化学成分（双香豆素、7-羟基-6-甲氧基香豆素、8-羟基补骨脂素、香豆素、6,7-二甲氧基香豆素、二氢香豆素、7-甲氧基香豆素、7-甲基香豆素、补骨脂素、8-甲氧基补骨脂素、5-甲氧基补骨脂素、4-甲基-7-乙氧基香豆素、2′,4,8-三甲基补骨脂素、欧前胡素、异欧前胡素、二氢欧山芹醇当归酸酯、环香豆素）的高效液相色谱分析方法。样品经甲醇超声提取,以8 000 r/min离心15 min,取上清液过滤后测定。采用Agilent Zorbax SB-Phenyl柱（4.6 mm×250 mm,5μm）分离,以水-甲醇-乙腈三元流动相梯度洗脱,流速1.0 mL/min,柱温30℃。采用二极管阵列检测器多波长检测（210、220、250 nm）,外标法定量。17种香豆素类化合物的定量下限（LOQ）为1 mg/kg,线性范围为0.5~60 mg/L,相关系数（r）均大于0.999。在高、中、低3种加标水平下的平均加标回收率（n=6）为86%~106%,相对标准偏差为0.3%~3.6%。该方法准确、简便,适用于化妆品中17种香豆素类化合物的测定。A high performance liquid chromatographic（HPLC） method was developed for the simultaneous determination of 17 coumarins,including dicoumarol,7-hydroxy-6-methoxy-coumarin,8-hydroxypsoralene thanxol,coumarin,6,7-dimethoxycoumarin,hydrocoumarin,7-methoxycoumarin,7-methylcoumarin,psoralen,8-methoxypsoralen,5-methoxypsoralen,4-methyl-7-ethoxycoumarin,2′,4,8-trimethylpsoralen,imperatorin,isoimperatorin,columbianadin and cyclocoumarol in cosmetics.The samples were extracted supersonically with methanol centrifuged at 8 000 r/min for 15 min.The supernatant was separated on an Aglient Zorbax SB-Phenyl column（4.6 mm × 250 mm,5 μm） by gradient elution with water-methanol-acetonitrile as mobile phase at a flow rate of 1.0 mL/min and column temperature of 30 ℃.The analysis of 17 coumarins was performed by HPLC with diode array detector at the wavelength of 210,220,250 nm,respectively.Identification of 17 coumarins was achieved by retention time,and quantification analysis was performed by the external standard method.The limits of quantitation（LOQ） for 17 coumarins were 1 mg/kg.The method showed a good linearity in the range of 0.5-60 mg/L with correlation coefficients more than 0.999.The mean recoveries at three spiked levels were in the range of 86%-106% with RSDs of 0.3%-3.6%.The method was accurate and simple,and was suitable for the determination of 17 coumarins in cosmetics samples.

关 键 词：高效液相色谱 香豆素类化合物 化妆品 

分 类 号：O657.72[理学—分析化学] O625.1[理学—化学]