三黄喷雾剂中黄芩、栀子提取物的大孔吸附树脂纯化工艺  

Purification Processes of Extract of Scutellaria and Gardenia from Sanhuang Spray with Macroporous Adsorption Resin

作　　者：朱鹏飞[1] 冯伟红[2] 刘淑芝[2] 

机构地区：[1]首都医科大学中医药学院,北京100069 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2010年第17期4-6,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：重大新药创制(2009ZX09502-010;2009ZX09301-005-05);中国中医科学院自主选题项目(Z02094)

摘　　要：目的:研究大孔吸附树脂纯化三黄喷雾剂中黄芩的工艺条件。方法:采用HPLC测定黄芩苷含量,以黄芩苷的吸附率、解吸率为考察指标,优选大孔吸附树脂最佳纯化工艺条件。结果:最佳大孔吸附树脂型号为SP-825;上样液浓度为0.2 g·mL-1,径高比为1∶7,最大上样量为2 BV,洗脱剂为70%乙醇,洗脱流速为3 BV·h-1,洗脱剂用量为6 BV。结论:SP-825型大孔吸附树脂纯化黄芩、栀子提取物的最佳工艺稳定、可行。Objective：To investigate the purification processes of Scutellaria from Sanhuang spray with macroporous adsorption resin. Method： The content of baicalin was measured with HPLC. The adsorption rate and desorption rate were measured to evaluate the optimal macroporous adsorption resin and the optimal purification conditions. Result： The optimal macroporous adsorption resin was SP-825. The optimal purification condition was as follows ： the sample concentration was 0.2g·mL^-1 ; the diameter height ratio was 1 ： 7 ; the sample volume was 2 BV; the eluant was 70% ethanol; the elution flow rate was 3 BV·h^-1; the ethanol consumption was 6 BV. Conclusion： Purification process of the extract of Scutellaria and Gardenia with SP-825 macroporous adsorption resin was stable and feasible.

关 键 词：大孔吸附树脂 黄芩苷 纯化 

分 类 号：R283.6[医药卫生—中药学]