通塞脉微丸高效毛细管电泳指纹图谱的研究  

Research on HPCE Fingerprint of Tongsaimai Pellet

作　　者：李伟东[1] 张志杰[2] 蔡宝昌[1] 

机构地区：[1]南京中医药大学江苏省中药炮制重点试验室,南京210029 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2010年第17期53-56,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家863重大研究计划项目(2003AA2Z3247);江苏省科技厅科技攻关重大项目(BK2001209)

摘　　要：目的:建立通塞脉微丸高效毛细管电泳指纹图谱。方法:以pH 9.18,40 mmol·L-1硼砂-10%甲醇溶液为运行缓冲液,采用未涂层弹性石英毛细管柱(75μm×56 cm,有效长度50.0 cm),分离电压25 kV;进样压力5 kPa,进样2.0 s;毛细管温度25℃;二极管阵列检测,检测波长254 nm。结果:以10个批次的通塞脉微丸为样品建立了通塞脉微丸的毛细管电泳指纹图谱,确认共有指纹峰16个,并确定了甘草苷、甘草素、甘草酸单铵盐、肉桂酸、绿原酸、阿魏酸的峰。结论:此方法简单、快速、高效,为通塞脉微丸的全面质量控制提供了科学依据。Objective：To establish an HPCE method for determining the fingerprint of Tongsaimai pellet.Method：The buffer was composed of 40 mmol·L-1borax（pH 9.18）-methanol（90∶ 10）.CE was performed on a 75 μm ×56 cm（effective length is 50.0 cm）fused silica capillary.Separation voltage was 25 kV,sampling pressure was 5 kPa,sampling time was 2.0 s,the temperature was maintained at 25 ℃,and detected at 254 nm.Result： Based on the fingerprints of 10 batches Tongsaimai pellet,an average electropherogram was used as the standard fingerprint,among which there were 16 characteristic peaks.Conclusion：The fingerprint analysis can be used for assessing the quality consistency of Tongsaimai pellet from batch to batch.

关 键 词：通塞脉微丸 指纹图谱 高效毛细管电泳 

分 类 号：R284.1[医药卫生—中药学]