肉桂水提物对正常大鼠尿液代谢物组的影响  

Metabomonics Study on Urine of Normal Rats Administered with Cinnamomi Cortex Water Extract

作　　者：张启云[1] 李冰涛[1] 徐国良[1] 孙立波[1] 汤喜兰[1] 黄丽萍[2] 余日跃[2] 刘红宁[1] 

机构地区：[1]江西中医学院现代中药制剂教育部重点试验室,南昌330006 [2]江西中医学院药学院,南昌330006

出　　处：《中国实验方剂学杂志》2010年第17期164-167,171,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划(973计划)基金项目(2006CB504702)

摘　　要：目的:研究肉桂水提物对大鼠尿液中内源性代谢产物的影响。方法:肉桂水提物0.3 g·kg-1大鼠连续ig 30 d,收集0,1,8,15,22,29 d尿样,LC-MS/MS采集样品数据,经主成分分析(PCA),正交信号校正偏最小二乘法判别分析(OSC-PLS-DA)处理数据,结合生物标记物和相关资料,分析肉桂水提物对大鼠内源性代谢产物的影响。结果:肉桂水提物ig 8 d对大鼠尿液中内源性物质的影响最大。与空白组比较,给药组尿样中CPA[18∶1(11Z)/0∶0],Gamma-linolenyl carnitine等物质浓度上升,Deoxyuridine,CerP(d18∶1/22∶0)等物质浓度降低。结论:肉桂水提物能显著影响大鼠体内与基础代谢和细胞信号相关物质的代谢。Objective：To investigate the changes of endogenous metabolites concentrations in the urine of normal rats administered with Cinnamomi Cortex water extract.Method： The administered group were administered orally with Cinnamomi Cortex water extract for 30 days.Urine was collected at 0,1,8,15,22,29 d after administration respectively and analyzed by LC /MS /MS.The acquired data were analyzed by PCA,OSC-PLS-DA.Result： After administration for 8 days,endogenous metabolites concentration in the urine of administered rats changed apparently.Through OSC-PLS-DA analysis,21 biomarkers were found.The identified biochemical effects of these biomarkers were associated with the pharmacological actions of Cinnamomi Cortex,including increased concentrations of CPA（18 ∶ 1（11Z） /0 ∶ 0）,gamma-linolenyl carnitine and decreased concentrations of deoxyuridine,CerP（d18∶ 1 /22∶ 0）.Conclusion： The administration of Cinnamomi Cortex water extract may change the cell signals and basic metabolism significantly in rat.

关 键 词：肉桂 代谢组学 生物标记物 

分 类 号：R285.5[医药卫生—中药学]