丙肝病毒F基因对c-myc转录调控作用  

Effect of HCV-F gene on transcription activation of c-myc promoter

作　　者：孔晶[1,2] 储春丽[1] 邓小昭[1] 张云[1] 李春雷[1] 韦娟[1] 刘燕[1] 

机构地区：[1]南京军区军事医学研究所疾病控制研究所,南京210002 [2]淮阴工学院生命科学与化学工程学院

出　　处：《中国公共卫生》2010年第12期1508-1510,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金面上项目(30972628);江苏省自然科学基金面上项目(BK2010112);江苏省高校自然科学研究项目(09KJB330001);中国博士后科学基金项目(20090451569);江苏省博士后科研资助计划项目(0901060C)

摘　　要：目的观察丙型肝炎病毒（HCV）F基因对c-myc启动子转录活性的影响,探讨HCV F基因表达产物参与肝细胞癌变的机制。方法构建c-myc启动子的萤光素酶报告质粒,与HCV-F和HCV-C的表达质粒共转染人肝癌细胞系HepG 2细胞,测定萤光素酶的活性,分析HCV-F、HCV-C对c-myc启动子转录活性的影响。结果在HepG 2细胞中,HCV-C对c-myc启动子的转录具有明显的促进作用,启动子的转录活性比对照组提高约7倍,且在HCV-C的剂量为2~10μg时,其作用具有剂量依赖性;HCV-F对c-myc启动子转录活性也具有激活作用,在剂量为2μg时,启动子的转录活性约为对照组的3倍,c-myc启动子对HCV-F的剂量依赖激活作用不明显。结论 HCV-F相比HCV-C不能有效的调节c-myc启动子转录活性,HCV-C起主要的调节功能。Objective To observe the effect of hepatitis C virus-F（HCV-F） gene on transcription activition of c-myc promoter and to explore the role of HCV-F on pathogenesis of hepatocarcinogenesis.Methods The luciferase reporter plasmids of c-myc promoter were constructed and cotransfected into HepG 2 cells with HCV-F and HCV-C expression plasmids.The transactivition of c-myc promoter was assayed by luminometer.Results HCV-C exhibited a distinct promotion activity to c-myc promoter,with a seven-folds increase of c-myc promoter transactivity compared to that of the control group.HCV-F showed less promotion activity and enhanced luciferase activity about three-folds increse of c-myc promoter.At a range of 2 to 10 μg,the enhancement of c-myc promoter was observed to be dose-dependent only with HCV-C not HCV-F.ConclusionHCV-F exhibits less promotion activity for c-myc promoter than HCV-C,which does not posses all functions of HCV-C protein.

关 键 词：丙型肝炎病毒(HCV) F基因 c-myc启动子 萤光素酶 

分 类 号：R512.63[医药卫生—内科学]