半胱氨酸蛋白酶-3在染砷大鼠肾近端小管表达  

Expression of caspase-3 in renal proximal convoluted tubules in rats with arsenic poisoning

作　　者：徐国强[1] 李远慧[1] 李娜[1] 钱立全[1] 

机构地区：[1]贵阳医学院生理学教研室,贵州贵阳550004

出　　处：《中国公共卫生》2010年第12期1550-1551,共2页Chinese Journal of Public Health

基　　金：贵州省科技计划项目(黔科合S字【2007】1059号)

摘　　要：目的探讨慢性染砷对大鼠肾脏近端小管上皮细胞半胱氨酸蛋白酶3(caspase-3)表达的影响。方法将30只健康成年清洁级SD大鼠随机分为高、低剂量(分别为10.0,0.4 mg/kg)染砷组和对照(蒸馏水)组,采用自由饮水方式(As2O3溶于饮水中)进行染毒,染毒容量为200 mL/kg,连续染毒6个月,测血砷、尿砷含量,取大鼠肾脏进行HE染色,免疫组化法检测染砷大鼠近端小管上皮细胞caspase-3的表达,图像分析计数。结果与对照组比较,高、低剂量染砷组大鼠血砷、尿砷明显增高(P<0.01);染砷大鼠近端小管上皮细胞空泡样变性,细胞腔面刷状缘脱落并减少,可见核固缩;与对照组(1.30±1.02)个/视野比较,高、低剂量染砷组大鼠肾近端小管上皮细胞caspase-3阳性细胞数分别为(63.94±10.83)、(16.00±8.23)个/视野,明显升高(P<0.05);高、低剂量染砷组大鼠肾近端小管上皮细胞caspase-3表达灰度值分别为(90.43±11.43)、(107.94±10.72),与对照组比较,差异均有统计学意义(P<0.05)。结论慢性砷中毒可致大鼠近端小管上皮细胞caspase-3表达。Objective To explore the effect of chronic arsenic poisoning on the caspase-3 expression in epithelium cells of proximal convoluted tubules in rats.Methods Thirty healthy adult SD rats in cleanliness grade were divided randomly into three groups：high and low dose arsenism group with arsenism of 10.0 and 0.4 mg/kg·d,respectively,and control group with distilled water.Drinking water was contaminated with As2O3.The rats were exposed to the contaiminated drinking water freely for six months.The content of arsenic in the blood and urine of the rats was determined.The change of configuration and expression of caspase-3 in epithelium cells of proximal convoluted tubules of the kidneys of arsenism rats were observed with HE,strept vidin-biotin complex（SABC） immunohistochemistry and image pattern analysis methods.Results Compared with the control group,a higher arsenic content in the blood and urine was found in both high and low dose arsenism group（P0.01）.Vacuole degeneration was found in epithelium cells and brush border of lumen surface fell off and reduced with pyknotic nucleus in proximal convoluted tubules of arsenism rats.The positive cells of caspase-3 was increased in control group（1.30±1.02）,low dose（16.00±8.23/area） and high dose（63.94±10.83/area）（P0.05）.While the grey scale was decreased significantly in high dose（107.94±10.72/area） and low dose（148.23±11.74/area） compared to that of the control group（P0.05）.Conclusion Chronic arsenic poisoning may induce the expression of caspase-3 in epithelium cells in proximal convoluted tubules in rats.

关 键 词：砷中毒 半胱氨酸蛋白酶3(caspase-3) 近端小管 凋亡 大鼠 

分 类 号：R995[医药卫生—毒理学]