bFGF对脑缺血大鼠海马及顶叶皮质c-Myc蛋白影响  

Effects of bFGF on expression of c-Myc in cerebral tissue during focal cerebral ischemia in rats

作　　者：吕威力[1] 邢雪松[1] 张国斌[1] 

机构地区：[1]沈阳医学院病理学教研室,辽宁110039

出　　处：《中国公共卫生》2010年第12期1566-1567,共2页Chinese Journal of Public Health

基　　金：辽宁省教育厅科学研究计划(05L442)

摘　　要：目的探讨碱性成纤维细胞生长因子(bFGF)对脑组织缺血再灌注神经元c-Myc蛋白表达影响及机制。方法 30只大鼠随机分为假手术组、缺血再灌注组、bFGF组,每组10只;应用线栓法制作大鼠局灶性脑缺血再灌注模型,大脑中动脉阻塞2 h再灌注损伤24 h,采用原位末端标记法(TUNEL)、免疫组织化学法检测海马及顶叶皮质内神经元凋亡和c-Myc蛋白表达。结果假手术组大鼠海马及顶叶皮质偶见凋亡细胞,神经元内少见c-Myc蛋白阳性细胞;缺血再灌注组海马及顶叶皮质神经元凋亡增加,顶叶皮质及海马神经元内c-Myc蛋白表达灰度值为(88.16±2.43),(86.72±1.23),bFGF组神经元凋亡减少,神经元内c-Myc蛋白表达灰度值为(97.61±1.78),(95.35±2.34)。结论 bFGF可减少缺血神经元凋亡,抑制脑缺血诱导的c-Myc蛋白的表达,对脑缺血再灌注海马及顶叶皮质神经元具有保护作用。Objective To investigate the expression of c-Myc in hippocampus and cortex after cerebral ischemia and reperfusion in rats and to explore regulative effects and mechanism of basic fibroblast growth factor（bFGF）to c-Myc in brain tissue.Methods The model of middle cerebral artery occlusion（MCAO） was established with intraluminal filament blockade.The expression of c-Myc and cell apoptosis in hippocampus and cortex were detected with immunohistochemical and terminal deoxyneucleotidyl transferase dUTP nick end labeling（TUNEL） method.Results No apoptotic cell was observed in sham-operation group.The expression of c-Myc in the cortex and hippocampus tissue of rats was at low level in the sham-operation group.In ischemia-reperfusion group,the apoptotic neurons in ischemic region of cortex and hippocampus were enhanced.The decrease of apoptotic neurons in ischemic region of cortex and hippocampus was observed in bFGF treatment group.The grayscale value of the expression of c-Myc was increased in the model group（88.16±2.43,86.72±1.23）.The grayscale value of the expression of c-Myc in the cortex and hippocampus tissue was markably decreased in the treatment group than that of in the model group（97.61±1.78,95.35±2.34）.Conclusion The results indicate that bFGF depress cell apoptosis,participate in the regulation of expression of c-Myc in ischemic neurons.

关 键 词：脑缺血 碱性成纤维细胞生长因子 C-MYC 海马 凋亡 

分 类 号：Q343[生物学—遗传学]