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作 者:江正君[1] 贾琳[2] 蒋磊[2] 李瑞[2] 檀根甲[2] 江彤[2]
机构地区:[1]安徽农业大学农业园及试验总场管理中心,合肥230036 [2]安徽农业大学植物保护学院,合肥230036
出 处:《中国农学通报》2010年第23期286-290,共5页Chinese Agricultural Science Bulletin
基 金:安徽省教育厅自然科学基金重点项目"辅助离子注入选育高效生防菌对苹果采后炭疽病的控制效果与作用机理研究"(KJ2007A095)
摘 要:为了明确漆树炭疽菌的种类及其病原菌生长特性,此研究采集感染炭疽病的漆树病叶,组织分离得到病原菌纯培养,根据病原菌的形态、培养特征、致病性和寄主范围将其鉴定为胶孢炭疽Colletotrichum gloeosporioides(Penz.)Sacc.。进一步研究其生物学特性发现,漆树炭疽菌菌丝生长与时间呈直线相关,菌丝在10~35℃范围内均能生长,生长最适温度为25~28℃,分生孢子致死温度为48℃,分生孢子在相对湿度达到90%时才能萌发,连续光照可以促进菌丝生长量和产孢量的增加,菌丝在pH3~12范围内均能生长,生长最适pH为6.0。漆树炭疽菌对多种碳、氮源都能利用,以葡萄糖、果糖为碳源以及以天门冬酰胺、丙氨酸为氮源时,其菌丝生长最佳。To ascertain the species of Lacquer anthracnose pathogen and its growth characteristics,Lacquer leaves infected with anthracnose disease were collected,and pure culture of the pathogen was acquired by tissue isolation method.The pathogen is determined to be Colletotrichum gloeosporioides (Penz.) Sacc.by its morphologic characters,cultural characteristics,pathogenicity and host range.Further research work on its biological characteristics was conducted.It was found that mycelial growth of Lacquer anthracnose pathogen had a linear correlation with the cultural time.The temperature range of the mycelial growth was 10~35℃,and the optimum temperature was 25~28℃.The lethal temperature of conidia was 48℃.The conidia cannot germinate until relative humidity reached 90%.Continuous light may promote the increasing of yield and sporulation of mycelia.The mycelia can grow at the range of pH 3~12 and the optimum growth pH was 6.0.The Lacquer anthracnose pathogen can utilize many kinds of carbon and nitrogen source.The mycelia will grow better if glucose,fruitose was used as the carbon sources and asparamide,alanine was used as the nitrogen sources.
分 类 号:S763.15[农业科学—森林保护学]
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